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    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation
    9.
    发明授权
    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation 有权

    公开(公告)号：US10144914B2

    公开(公告)日：2018-12-04

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/00

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION
    10.
    发明申请
    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION 审中-公开
    用于保护肽固定表面的改性基质从伽马辐射降解

    公开(公告)号：US20110207219A1

    公开(公告)日：2011-08-25

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N2533/20 ,  C12N2533/50

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    摘要翻译： 在本说明书和权利要求书中，公开了三种修饰的细胞培养基质，用于保护细胞培养技术中使用的肽模拟表面。 该方法能够保留与表面缀合的生物活性物质的功能。 特别地，玻连蛋白肽片段Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2能够促进未分化人的生长和增殖 使用3种不同的修饰的细胞培养物底物进行稳定后的胚胎干细胞以防止γ照射。 本发明公开的改性底物是（i）抗氧化剂分子的共价连接（通过封闭步骤）; （ii）使用包含抗氧化剂和稳定剂的涂层，其包含糖调节剂，糖，碳水化合物，聚（氨基酸），肽和亲水性聚合物; 和（iii）第三种方法，其中用于促进人类胚胎干细胞生长和增殖的生物活性序列中所含的相同甲硫氨酸也用作牺牲涂层以保护相同缀合序列免受γ照射的损伤。