公开(公告)号：US07087896B2

公开(公告)日：2006-08-08

申请号：US11023234

申请日：2004-12-27

申请人： Christopher H. Becker ,  Curtis A. Hastings ,  Scott M. Norton ,  Sushmita Mimi Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Andrew Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

发明人： Christopher H. Becker ,  Curtis A. Hastings ,  Scott M. Norton ,  Sushmita Mimi Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Andrew Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/0036

摘要： Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

摘要翻译： 通过对光谱进行归一化，可以从质谱中获得关于化学或生物样品成分的相对定量信息，以产生准确反映负责物种浓度的峰值强度值。 归一化因子是由浓度在一系列样品中保持不变的那些固有成分的峰值强度计算出来的。 发生在不同样品中的组分的相对浓度可以从标准化峰强度估计。 与传统方法不同，不需要内标或附加试剂。 该方法对于蛋白质组学和代谢组学研究中的差异表型特别有用，其中鉴定了样品浓度变化的分子。 这些鉴定的物种可以作为疾病或治疗反应的生物标志物。

公开(公告)号：US20050116159A1

公开(公告)日：2005-06-02

申请号：US11023234

申请日：2004-12-27

申请人： Christopher Becker ,  Curtis Hastings ,  Scott Norton ,  Sushmita Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

发明人： Christopher Becker ,  Curtis Hastings ,  Scott Norton ,  Sushmita Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

IPC分类号： H01J49/04 ,  H01J49/00

CPC分类号： H01J49/0036

摘要： Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

摘要翻译： 通过对光谱进行归一化，可以从质谱中获得关于化学或生物样品成分的相对定量信息，以产生准确反映负责物种浓度的峰值强度值。 归一化因子是由浓度在一系列样品中保持不变的那些固有成分的峰值强度计算出来的。 发生在不同样品中的组分的相对浓度可以从标准化峰强度估计。 与传统方法不同，不需要内标或附加试剂。 该方法对于蛋白质组学和代谢组学研究中的差异表型特别有用，其中鉴定了样品浓度变化的分子。 这些鉴定的物种可以作为疾病或治疗反应的生物标志物。