公开(公告)号：US07087896B2

公开(公告)日：2006-08-08

申请号：US11023234

申请日：2004-12-27

申请人： Christopher H. Becker ,  Curtis A. Hastings ,  Scott M. Norton ,  Sushmita Mimi Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Andrew Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

发明人： Christopher H. Becker ,  Curtis A. Hastings ,  Scott M. Norton ,  Sushmita Mimi Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Andrew Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/0036

摘要： Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

摘要翻译： 通过对光谱进行归一化，可以从质谱中获得关于化学或生物样品成分的相对定量信息，以产生准确反映负责物种浓度的峰值强度值。 归一化因子是由浓度在一系列样品中保持不变的那些固有成分的峰值强度计算出来的。 发生在不同样品中的组分的相对浓度可以从标准化峰强度估计。 与传统方法不同，不需要内标或附加试剂。 该方法对于蛋白质组学和代谢组学研究中的差异表型特别有用，其中鉴定了样品浓度变化的分子。 这些鉴定的物种可以作为疾病或治疗反应的生物标志物。

公开(公告)号：US20050116159A1

公开(公告)日：2005-06-02

申请号：US11023234

申请日：2004-12-27

申请人： Christopher Becker ,  Curtis Hastings ,  Scott Norton ,  Sushmita Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

发明人： Christopher Becker ,  Curtis Hastings ,  Scott Norton ,  Sushmita Roy ,  Weixun Wang ,  Haihong Zhou ,  Thomas Shaler ,  Praveen Kumar ,  Markus Anderle ,  Hua Lin

IPC分类号： H01J49/04 ,  H01J49/00

CPC分类号： H01J49/0036

摘要： Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

摘要翻译： 通过对光谱进行归一化，可以从质谱中获得关于化学或生物样品成分的相对定量信息，以产生准确反映负责物种浓度的峰值强度值。 归一化因子是由浓度在一系列样品中保持不变的那些固有成分的峰值强度计算出来的。 发生在不同样品中的组分的相对浓度可以从标准化峰强度估计。 与传统方法不同，不需要内标或附加试剂。 该方法对于蛋白质组学和代谢组学研究中的差异表型特别有用，其中鉴定了样品浓度变化的分子。 这些鉴定的物种可以作为疾病或治疗反应的生物标志物。

公开(公告)号：US20100015717A1

公开(公告)日：2010-01-21

申请号：US12391119

申请日：2009-02-23

申请人： Rajendra Singh ,  Haihong Zhou

发明人： Rajendra Singh ,  Haihong Zhou

IPC分类号： G01N33/68 ,  C07K2/00 ,  C07D235/02 ,  C07D401/00 ,  C07D409/00

CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07D495/04 ,  G01N33/6851

摘要： The present invention relates generally to novel protein modification reagents for fractionation and quantitative (differential) profiling of proteins in a complex mixture. The reagents react with amino acids or other protein components or structures and function as mass tags. The present invention provides methods of making the protein modification reagents and methods of using the protein modification reagents for quantitative analysis of proteins.

摘要翻译： 本发明一般涉及用于复合混合物中蛋白质的分级和定量（差异）分布的新型蛋白质修饰试剂。 试剂与氨基酸或其他蛋白质组分或结构物反应，起到质量标签的作用。 本发明提供了制备蛋白质修饰试剂的方法和使用蛋白质修饰试剂进行蛋白质定量分析的方法。

公开(公告)号：US20060008856A1

公开(公告)日：2006-01-12

申请号：US11220746

申请日：2005-09-06

申请人： Rajendra Singh ,  Haihong Zhou

发明人： Rajendra Singh ,  Haihong Zhou

IPC分类号： G01N33/53 ,  C07K14/47

CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07D495/04 ,  G01N33/6851

摘要： The present invention relates generally to novel protein modification reagents for fractionation and quantitative (differential) profiling of proteins in a complex mixture. The reagents react with amino acids or other protein components or structures and function as mass tags. The present invention provides methods of making the protein modification reagents and methods of using the protein modification reagents for quantitative analysis of proteins.

公开(公告)号：US06476118B1

公开(公告)日：2002-11-05

申请号：US09507496

申请日：2000-02-18

申请人： Barry L. Karger ,  Lev Kotler ,  Wolfgang Goetzinger ,  Haihong Zhou ,  Zoran Sosic ,  Oscar Salas-Solano

发明人： Barry L. Karger ,  Lev Kotler ,  Wolfgang Goetzinger ,  Haihong Zhou ,  Zoran Sosic ,  Oscar Salas-Solano

IPC分类号： C08L3104

CPC分类号： G01N27/44747 ,  C08L33/24 ,  C08L33/26 ,  C08L2205/02 ,  C08L2666/04 ,  C08L33/00

摘要： A method of separating a mixture of biopolymers of various lengths using a blend of two linear hydrophilic polymers, particularly polyacrylamides (LPA) or substituted polyacrylamides, one with high weight-average molecular mass and another with low weight-average molecular mass, each present at a low concentration but wherein the concentration of the high weight-average molecular mass LPA is above its entanglement threshold, is disclosed. The matrix is excellent for use in methods of nucleic acid analysis, particularly long read length DNA sequencing.

摘要翻译： 使用两种直链亲水性聚合物，特别是聚丙烯酰胺（LPA）或取代的聚丙烯酰胺（一种具有高重均分子量，另一种具有低重均分子量）的共混物的混合物分离各种长度的生物聚合物的混合物的方法， 公开了低浓度，但其中高重均分子量LPA的浓度高于其缠结阈值。 该基质非常适用于核酸分析方法，特别是长读长度DNA测序。