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1.发明授权Compositions and methods relating to elutable carbohydrate-binding proteins 失效与可消除碳水化合物结合蛋白有关的组合物和方法公开(公告)号：US08288337B2
公开(公告)日：2012-10-16
申请号：US12399075
申请日：2009-03-06
CPC分类号： C07K14/32 , C07K14/39 , G01N2333/32 , G01N2333/39
摘要： Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.
摘要翻译： 提供了组合物和方法，用于在天然蛋白质保持结合的条件下产生和鉴定可逆结合碳水化合物底物的突变碳水化合物结合蛋白。 提供了修饰的几丁质结合结构域的实例，其可以在还原剂的存在下或在5-10的范围内的pH下从几丁质中洗脱出来。
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2.发明申请Compositions and Methods Relating to Elutable Carbohydrate-Binding Proteins 失效与可补充的碳水化合物结合蛋白相关的组合物和方法公开(公告)号：US20090247423A1
公开(公告)日：2009-10-01
申请号：US12399075
申请日：2009-03-06
IPC分类号： C40B30/04
CPC分类号： C07K14/32 , C07K14/39 , G01N2333/32 , G01N2333/39
摘要： Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.
摘要翻译： 提供了组合物和方法，用于在天然蛋白质保持结合的条件下产生和鉴定可逆结合碳水化合物底物的突变碳水化合物结合蛋白。 提供了修饰的几丁质结合结构域的实例，其可以在还原剂的存在下或在5-10的范围内的pH下从几丁质中洗脱出来。
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3.发明授权Compositions and methods relating to elutable carbohydrate-binding proteins 失效与可消除碳水化合物结合蛋白有关的组合物和方法公开(公告)号：US07732565B2
公开(公告)日：2010-06-08
申请号：US11524015
申请日：2006-09-20
IPC分类号： C07K14/00
CPC分类号： C07K14/32 , C07K14/39 , G01N2333/32 , G01N2333/39
摘要： Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.
摘要翻译： 提供了组合物和方法，用于在天然蛋白质保持结合的条件下产生和鉴定可逆结合碳水化合物底物的突变碳水化合物结合蛋白。 提供了修饰的几丁质结合结构域的实例，其可以在还原剂的存在下或在5-10的范围内的pH下从几丁质中洗脱出来。
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公开(公告)号：US08647855B2
公开(公告)日：2014-02-11
申请号：US13129482
申请日：2009-11-16
CPC分类号： C12Y305/01025 , C12N9/1205 , C12N9/78 , C12N9/80 , C12P7/06 , C12P7/08 , C12Y207/01059 , C12Y305/99006 , Y02E50/17
摘要： Compositions and methods are provided for generating biofuels by fermentation from carbon sources other than glucose using genetically engineered yeast strains. For example, a Saccharomyces strain which is capable of converting glucose to ethanol but not of metabolizing N-acetyl glucosamine is genetically engineered to utilize N-acetyl glucosamine as a nutrient carbon source.
摘要翻译： 提供了组合物和方法，用于通过使用遗传工程酵母菌株从除葡萄糖以外的碳源发酵生成生物燃料。 例如，能够将葡萄糖转化为乙醇但不能代谢N-乙酰葡糖胺的酵母属菌株被遗传工程化以利用N-乙酰葡糖胺作为营养碳源。
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5.发明授权rBSA from K. lactis expression, secretion and purification of recombinant bovine serum albumin (rBSA) from K. lactis and uses thereof 失效乳酸克鲁维酵母的rBSA表达，乳酸克鲁维酵母重组牛血清白蛋白（rBSA）的分泌和纯化及其用途公开(公告)号：US08361750B2
公开(公告)日：2013-01-29
申请号：US12294759
申请日：2007-03-30
IPC分类号： C12P21/00 , C07K14/765 , C12N9/16
CPC分类号： C07K14/765 , C12N9/22 , C12N9/96 , C12N15/815
摘要： A recombinant BSA (rBSA) that (a) substantially lacks deoxyribonuclease activity as determined by incubating rBSA with linear DNA overnight and gel electrophoresis, (b) lacks animal viruses associated with animal-derived cell growth supplements; and (c) is capable of stabilizing DNA proteins is provided. Methods for making the rBSA and using it to stabilize enzymes are also provided.
摘要翻译： 重组BSA（rBSA），其（a）通过将rBSA与线性DNA一起孵育过夜和凝胶电泳确定，基本上缺乏脱氧核糖核酸酶活性，（b）缺少与动物来源的细胞生长补充物相关的动物病毒; 和（c）能提供稳定的DNA蛋白质。 还提供了制备rBSA并用于稳定酶的方法。
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公开(公告)号：US20120107877A1
公开(公告)日：2012-05-03
申请号：US13346258
申请日：2012-01-09
IPC分类号： C12P21/00
CPC分类号： C12P21/02 , C07K14/39 , C12N15/81 , C12N2800/101 , C12N2800/102 , C12N2820/702
摘要： Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
摘要翻译： 描述了与获得分泌的重组蛋白质的浓缩制剂相关的方法和组合物。 这些蛋白质以融合蛋白的形式与几丁质结合结构域（CBD）表达。 融合蛋白能够在几丁质的存在下被浓缩。 还描述了：包含经修饰的LAC4启动子的穿梭载体; 几丁质酶阴性宿主细胞; 一种能够在非变性条件下从几丁质中洗脱的CBD; 和灭菌的几丁质，其可以任选地被磁化以促进重组蛋白的回收。
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7.发明申请公开(公告)号：US20110071280A1
公开(公告)日：2011-03-24
申请号：US12399059
申请日：2009-03-06
CPC分类号： C12P21/02 , C07K14/39 , C12N15/81 , C12N2800/101 , C12N2800/102 , C12N2820/702
摘要： Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
摘要翻译： 描述了与获得分泌的重组蛋白质的浓缩制剂相关的方法和组合物。 这些蛋白质以融合蛋白的形式与几丁质结合结构域（CBD）表达。 融合蛋白能够在几丁质的存在下被浓缩。 还描述了：包含经修饰的LAC4启动子的穿梭载体; 几丁质酶阴性宿主细胞; 一种能够在非变性条件下从几丁质中洗脱的CBD; 和灭菌的几丁质，其可以任选地被磁化以促进重组蛋白的回收。
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公开(公告)号：US07060465B2
公开(公告)日：2006-06-13
申请号：US11235009
申请日：2005-09-26
IPC分类号： C12P21/00
CPC分类号： C12N15/80 , C12N9/2442 , C12Y302/01014
摘要： Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.
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9.发明申请Solubilization and Purification of a Target Protein Fused to a Mutant Maltose-Binding Protein 失效融合到突变体麦芽糖结合蛋白的靶蛋白的溶解和纯化公开(公告)号：US20120028305A1
公开(公告)日：2012-02-02
申请号：US13259837
申请日：2009-04-01
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility, The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consist of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
摘要翻译： 提供了与包含修饰的麦芽糖结合蛋白（MBP）的组合物相关的方法和组合物，当与蛋白质融合时，与蛋白质融合的野生型MBP相比，与蛋白质融合导致与麦芽糖糊精结合亲和力的增加，修饰的MBP 维持增加的溶解度。该修饰包括选自以下的突变：F68L，I318V，Q326R，V344M和T372TTTITITTTLGIEGR387，或由两个或更多个选自F68L，S146T，A313V，I318V，I318A， Q326R，V344M和T372TTTITITTTLGIEGR387突变体。
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10.发明授权公开(公告)号：US07517671B2
公开(公告)日：2009-04-14
申请号：US11242553
申请日：2005-10-03
IPC分类号： C12P21/02
CPC分类号： C12P21/02 , C07K14/39 , C12N15/81 , C12N2800/101 , C12N2800/102 , C12N2820/702
摘要： Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
摘要翻译： 描述了与获得分泌的重组蛋白质的浓缩制剂相关的方法和组合物。 这些蛋白质以融合蛋白的形式与几丁质结合结构域（CBD）表达。 融合蛋白能够在几丁质的存在下被浓缩。 还描述了：包含经修饰的LAC4启动子的穿梭载体; 几丁质酶阴性宿主细胞; 一种能够在非变性条件下从几丁质中洗脱的CBD; 和灭菌的几丁质，其可以任选地被磁化以促进重组蛋白的回收。
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