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公开(公告)号:US06175058B1
公开(公告)日:2001-01-16
申请号:US09263128
申请日:1999-03-05
申请人: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号: A01H100
CPC分类号: C12N9/00 , C12N15/8213 , C12N15/8289
摘要: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译: 在单子叶植物中,特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号:US06720475B1
公开(公告)日:2004-04-13
申请号:US09641111
申请日:2000-08-17
申请人: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号: A01H500
CPC分类号: C12N15/8289 , C12N9/00 , C12N15/8213
摘要: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译: 在单子叶植物中,特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号:US5929301A
公开(公告)日:1999-07-27
申请号:US972258
申请日:1997-11-18
申请人: Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
发明人: Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
CPC分类号: C12N9/00 , C12N15/8213 , C12N15/8289
摘要: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译: 在单子叶植物中,特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号:US06905857B2
公开(公告)日:2005-06-14
申请号:US10246581
申请日:2002-09-18
申请人: Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人: Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
CPC分类号: C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要: Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译: 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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公开(公告)号:US06541684B1
公开(公告)日:2003-04-01
申请号:US09246963
申请日:1999-02-09
申请人: Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人: Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
IPC分类号: A01H500
CPC分类号: C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要: Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译: 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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