Agrobacterium mediated transformation of sorghum
    7.
    发明授权
    Agrobacterium mediated transformation of sorghum 失效
    农杆菌介导的高粱转化

    公开(公告)号:US06369298B1

    公开(公告)日:2002-04-09

    申请号:US09056418

    申请日:1998-04-07

    IPC分类号: A01H100

    CPC分类号: C12N15/8205

    摘要: Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.

    摘要翻译: 提供了高效转化高粱的方法和组成。 该方法涉及用农杆菌感染,特别是包含超二元载体的那些。 以这种方式,可以将任何感兴趣的基因引入高粱植物中。 转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。 还提供了转化的高粱,细胞,组织,植物和种子。 本发明包括再生的,可育的高粱植物,由其产生的转基因种子,T1和后代。

    Method for regulating agrobacterium-mediated transformation
    8.
    发明授权
    Method for regulating agrobacterium-mediated transformation 有权
    调节农杆菌介导转化的方法

    公开(公告)号:US08143484B2

    公开(公告)日:2012-03-27

    申请号:US12357284

    申请日:2009-01-21

    IPC分类号: C12N15/84

    CPC分类号: C12N15/8205

    摘要: A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.

    摘要翻译: 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤,以产生活性的,通常为酚的化合物,并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达,从而转化从土壤杆菌到谷物细胞的T-DNA转移。

    Highly transformable elite doubled haploid line PH17AW
    9.
    发明授权
    Highly transformable elite doubled haploid line PH17AW 有权
    高度可转化的精英双倍体单倍体PH17AW

    公开(公告)号:US08030556B2

    公开(公告)日:2011-10-04

    申请号:US12174812

    申请日:2008-07-17

    CPC分类号: A01H5/10

    摘要: A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.

    摘要翻译: 一种新型双倍体玉米品系,命名为PH17AW,其种子,植物和植物部分。 用于生产玉米植物的方法,其包括用另一种玉米植物杂交双倍单倍体玉米品系PH17AW。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗入PH17AW的一种或多种性状,以及由此产生的玉米种子,植物和植物部分。 杂交玉米种子,植物或植物部分通过双重单倍体系PH17AW或PH17AW与另一个玉米品系的转化而产生。 衍生自双倍体单倍体玉米品系PH17AW的近交玉米品系,从双倍体单倍体玉米品系PH17AW生产其他近交玉米品系的方法和由这些方法衍生的近交玉米品系及其部分。

    Highly transformable elite inbred line-PHWWE
    10.
    发明授权
    Highly transformable elite inbred line-PHWWE 有权
    高度可转型的精英近交系 - PHWWE

    公开(公告)号:US07999158B2

    公开(公告)日:2011-08-16

    申请号:US12534507

    申请日:2009-08-03

    CPC分类号: A01H5/10

    摘要: A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.

    摘要翻译: 一种新型近交玉米品系被指定为PHWWE及其种子,植物和植物部分。 用于生产玉米植物的方法,其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状,以及由此生产的玉米种子,植物和植物部分。 杂交玉米种子,植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系,由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。