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公开(公告)号：US06905857B2
公开(公告)日：2005-06-14
申请号：US10246581
申请日：2002-09-18
申请人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
CPC分类号： C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译： 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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公开(公告)号：US06541684B1
公开(公告)日：2003-04-01
申请号：US09246963
申请日：1999-02-09
申请人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
发明人： Benjamin A. Bowen , Mark A. Chamberlin , Bruce J. Drummond , John A. McElver , Rodney J. Rothstein
IPC分类号： A01H500
CPC分类号： C12N9/00 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.
摘要翻译： 提供了编码在玉米植物中活性的两种RAD51重组酶的核酸序列。 还提供了包括可用作RFLP探针的ZmRAD51编码序列和独特的3'-非翻译区的cDNA序列。 还公开了含有编码ZmRAD51融合蛋白的核酸序列的质粒的产生以及使用质粒将ZmRAD51编码序列引入宿主细胞如玉米细胞的方法。
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3.发明授权Transposable element-anchored, amplification method for isolation and identification of tagged genes 失效可转移元件锚定，用于分离和鉴定标记基因的扩增方法公开(公告)号：US06881539B1
公开(公告)日：2005-04-19
申请号：US09622353
申请日：1999-02-16
CPC分类号： C12N15/1065 , C12N15/1079 , C12N15/1082
摘要： A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.
摘要翻译： 提供了用于快速分离和鉴定转座元件标记基因的DNA序列的方法。 该方法包括使用可转座元件锚定扩增来鉴定和克隆与突变表型相关的扩增产物的修饰AFLP方法。 一旦被克隆，可以使用目的扩增产物直接筛选cDNA文库或进行测序，并与序列同源性的可用数据库进行比较。 该方法的修改提供了用于将额外类型的插入事件的位置识别到基因组DNA中的方法，更具体地说是将基因插入到宿主生物体的基因组中。
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公开(公告)号：US06175058B1
公开(公告)日：2001-01-16
申请号：US09263128
申请日：1999-03-05
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H100
CPC分类号： C12N9/00 , C12N15/8213 , C12N15/8289
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译： 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US5929301A
公开(公告)日：1999-07-27
申请号：US972258
申请日：1997-11-18
申请人： Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
发明人： Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
CPC分类号： C12N9/00 , C12N15/8213 , C12N15/8289
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译： 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US06720475B1
公开(公告)日：2004-04-13
申请号：US09641111
申请日：2000-08-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H500
CPC分类号： C12N15/8289 , C12N9/00 , C12N15/8213
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译： 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US20110030107A1
公开(公告)日：2011-02-03
申请号：US12909944
申请日：2010-10-22
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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公开(公告)号：US06670189B2
公开(公告)日：2003-12-30
申请号：US09731393
申请日：2000-12-05
申请人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
发明人： Jonathan Duvick , Joyce R. Maddox , Tracy A. Rood , Xun Wang , Benjamin A. Bowen , Jacob T. Gilliam
IPC分类号： C12N1582
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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公开(公告)号：US06528700B1
公开(公告)日：2003-03-04
申请号：US09193612
申请日：1998-11-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N1582
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译： 提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。 该方法包括将嵌合寡核苷酸引入具有中间DNA残基的RNA残基中。 RNA嵌段与植物核苷酸序列同源。 嵌合寡核苷酸能折叠形成双链寡核苷酸。 嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。
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公开(公告)号：US06198020B1
公开(公告)日：2001-03-06
申请号：US09257541
申请日：1999-02-24
IPC分类号： A01H100
CPC分类号： C12N9/0075 , C07K14/415 , C07K14/47 , C12N15/8243 , C12N15/8279
摘要： Compositions and methods to aid in protecting the plant from invading pathogenic organisms are provided. The compositions of the invention comprise genes that influence the levels of nitric oxide in plant cells. Such genes include those encoding nitric oxide synthase (iNOS) as well as natural resistance-associated macrophage proteins (NRAMP) and NRAMP homologues. A pathogen inducible promoter or alternatively a constitutive, preferably a weak constitutive, promoter is used to control the desired level of disease control in the plant. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.
摘要翻译： 提供了有助于保护植物免受入侵病原生物体的组合物和方法。 本发明的组合物包含影响植物细胞中一氧化氮水平的基因。 这些基因包括编码一氧化氮合酶（iNOS）以及天然抗性相关巨噬细胞蛋白（NRAMP）和NRAMP同源物的基因。 病原体诱导型启动子或选择性的组成型优选弱组成型启动子用于控制植物中所需的疾病控制水平。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。
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