公开(公告)号：US20060121531A1

公开(公告)日：2006-06-08

申请号：US10523174

申请日：2003-05-22

申请人： Chuanzhong Wei ,  ZHIHONG Mo ,  Guiping Ma ,  Ping Jin ,  Bingling Li ,  Xuelong Tian ,  HAIYAN Tian ,  Gang Guo

发明人： Chuanzhong Wei ,  ZHIHONG Mo ,  Guiping Ma ,  Ping Jin ,  Bingling Li ,  Xuelong Tian ,  HAIYAN Tian ,  Gang Guo

IPC分类号： G01N33/53 ,  C12M1/34 ,  H01L21/00

CPC分类号： G01N33/6896 ,  G01N2800/2828

摘要： The present invention relates to the device for animal inspection and quarantine and the preparation method thereof. The present invention is especially applicable to the detection of the pathogen of bovine spongiform encephalopathy (BSE) (also known as “mad cow disease”). The present invention employs a piezoelectric chip, a microelectrode array and a common electrode fixed on the lower side surface and upper side surface of the piezoelectric chip, respectively, and a BSE PrP antibody array to constitute the piezoelectric biochip for the detection of the BSE pathogen. The BSE PrP antibodies are immobilized on the electrodes of the microelectrode array in a format corresponding uniquely to the electrodes of the microelectrode array by adsorbing, bonding, cross-linking, embedding or self-assembly process. The combination of the piezoelectric biochip and a detector constitutes the piezoelectric biochip detection system for the BSE pathogen. When the antibodies react with the corresponding PrPs immunochemically, the information about the PrPs can be detected at real time by measuring the resonant frequency, and the PrPs thus can be analyzed qualitatively and quantitatively. The present invention is applicable to the early, effective and rapid detection of the BSE pathogen.

摘要翻译： 本发明涉及动物检疫检疫装置及其制备方法。 本发明特别适用于牛海绵状脑病（BSE）病原体的检测（又称“疯牛病”）。 本发明分别采用固定在压电芯片的下侧表面和上侧表面上的压电芯片，微电极阵列和公共电极以及BSE PrP抗体阵列，以构成用于检测BSE病原体的压电生物芯片 。 通过吸附，结合，交联，嵌入或自组装过程，BSE PrP抗体以与微电极阵列的电极唯一相对应的格式固定在微电极阵列的电极上。 压电生物芯片和检测器的组合构成了BSE病原体的压电生物芯片检测系统。 当抗体与相应的PrPs免疫化学反应时，可以通过测量共振频率实时检测关于PrPs的信息，因此可以定性和定量地分析PrPs。 本发明适用于早期有效快速检测BSE病原体。