公开(公告)号：US20150099712A1

公开(公告)日：2015-04-09

申请号：US14399960

申请日：2013-05-09

申请人： Chun Liang ,  Zhihong Jiang ,  Ziyi Wang ,  Zhiling Yu ,  Jingrong Wang ,  Liping Bai

发明人： Chun Liang ,  Zhihong Jiang ,  Ziyi Wang ,  Zhiling Yu ,  Jingrong Wang ,  Liping Bai

IPC分类号： A61K31/7048 ,  G01N33/50 ,  G01N33/574 ,  A61K31/585

CPC分类号： A61K31/7048 ,  A61K31/585 ,  G01N33/5011 ,  G01N33/5044 ,  G01N33/574 ,  G01N2458/00 ,  G01N2500/10

摘要： A method for treating cancer by using an agent which is capable of inhibiting the functionality of the MCM complex, a heterohexameric ring formed from six subunits, in the process of DNA replication and a method of screening for such agents by detecting the locations and functions of the MCM subunits, such as hMcm2 and hMcm6, in cells treated with candidate compounds.

摘要翻译： 一种通过使用能够抑制MCM复合物的功能的试剂，由六个亚基形成的异六氢环在DNA复制过程中治疗癌症的方法以及通过检测这些试剂的位置和功能来筛选这些试剂的方法 MCM亚基，如hMcm2和hMcm6，在用候选化合物处理的细胞中。

公开(公告)号：US07393950B2

公开(公告)日：2008-07-01

申请号：US10232923

申请日：2002-08-29

申请人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

发明人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

IPC分类号： C07H21/04 ,  A61K31/70

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/315

摘要： Antisense oligonucleotides that inhibit expression of human replication-initiation protein as well as methods of preventing or treating hyperproliferative conditions using said oligonucleotides are disclosed. One aspect provides an antisense oligonucleotides that inhibits the expression of human replication-initiation protein and has a sequence complementary to at least a portion of a target sequence encoding a human replication-initiation gene. By administering a therapeutically effective amount of antisense oligonucleotides or by contacting the hyperproliferating cells with an effective amount of antisense oligonucleotides, expression of replication-initiation protein is inhibited. Methods of screening and testing active antisense oligonucleotides for their ability to inhibit gene expression are also disclosed.

摘要翻译： 公开了抑制人复制起始蛋白表达的反义寡核苷酸以及使用所述寡核苷酸预防或治疗过度增殖病症的方法。 一方面提供了抑制人复制起始蛋白的表达的反义寡核苷酸，并且具有与编码人复制起始基因的靶序列的至少一部分互补的序列。 通过施用治疗有效量的反义寡核苷酸或通过使过度增殖细胞与有效量的反义寡核苷酸接触，抑制复制起始蛋白的表达。 还公开了筛选和测试活性反义寡核苷酸抑制基因表达能力的方法。

公开(公告)号：US08318922B2

公开(公告)日：2012-11-27

申请号：US12126862

申请日：2008-05-24

申请人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

发明人： Chun Liang ,  Dao-rong Feng ,  Zhi-ling Yu

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H2/04

CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/315

摘要： Antisense oligonucleotides that inhibit expression of human replication-initiation protein as well as methods of preventing or treating hyperproliferative conditions using said oligonucleotides are disclosed. One aspect provides an antisense oligonucleotide that inhibits the expression of human replication-initiation protein and has a sequence complementary to at least a portion of a target sequence encoding a human replication-initiation gene. By administering a therapeutically effective amount of an antisense oligonucleotide or by contacting the hyperproliferating cells with an effective amount of one or more antisense oligonucleotides, expression of replication-initiation protein is inhibited. Methods of screening and testing active antisense oligonucleotides for their ability to inhibit gene expression are also disclosed.

摘要翻译： 公开了抑制人复制起始蛋白表达的反义寡核苷酸以及使用所述寡核苷酸预防或治疗过度增殖病症的方法。 一方面提供了抑制人复制起始蛋白的表达的反义寡核苷酸，并且具有与编码人复制起始基因的靶序列的至少一部分互补的序列。 通过施用治疗有效量的反义寡核苷酸或通过使过度增殖细胞与有效量的一种或多种反义寡核苷酸接触，抑制复制起始蛋白的表达。 还公开了筛选和测试活性反义寡核苷酸抑制基因表达能力的方法。

公开(公告)号：US20050042604A1

公开(公告)日：2005-02-24

申请号：US10909445

申请日：2004-08-02

申请人： Sun-Wing Tong ,  Chun Liang

发明人： Sun-Wing Tong ,  Chun Liang

IPC分类号： C12Q1/04 ,  C12Q1/70 ,  G01N33/554 ,  G01N33/569

CPC分类号： C12Q1/04

摘要： The present invention relates to a method of determining whether a facility can effectively prevent the spread of an infectious disease by a) releasing an effective amount of biological tracers in or near the facility, to mimic the spread of pathogens that cause the infectious disease; and b) detecting the presence or amount of the biological tracers to determine the spread of the biological tracers in the environment of the facility. The present invention further provides a method and apparatus to generate aerosol that mimic the human sneeze and cough so as to more realistically determine the spread of an infectious disease by such a source.

摘要翻译： 本发明涉及一种确定设施是否能有效防止传染病传播的方法：a）在设施内或附近释放有效量的生物示踪剂，以模拟导致感染性疾病的病原体的扩散; 和b）检测生物示踪剂的存在或数量以确定生物示踪剂在设施环境中的扩散。 本发明还提供了一种产生模拟人类打喷嚏和咳嗽的气溶胶的方法和装置，以更真实地确定这种来源的传染病的传播。