公开(公告)号：US08269063B2

公开(公告)日：2012-09-18

申请号：US10591419

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/10 ,  C07H21/04

CPC分类号： C12N9/12 ,  C12N15/8245 ,  C12Q1/485 ,  G01N33/573 ,  G01N2400/14 ,  G01N2500/04

摘要： The present invention relates to a method for identifying proteins involved in the phosphorylation of starch and nucleic acids which code for such proteins. The present invention further relates to plant cells and plants which exhibit an altered activity of a protein which can be identified using the method according to the invention. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of this starch and to the manufacture of starch derivatives of this modified starch.

摘要翻译： 本发明涉及一种鉴定涉及淀粉磷酸化的蛋白质的方法以及编码这种蛋白质的核酸。 本发明还涉及使用本发明方法鉴定的蛋白质活性变化的植物细胞和植物。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉以及制备该淀粉的方法和该淀粉衍生物的制造。

公开(公告)号：US07772463B2

公开(公告)日：2010-08-10

申请号：US10591428

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/29 ,  C12N15/52 ,  C12N15/63 ,  A01H5/00

CPC分类号： B02C9/00 ,  C12N9/12 ,  C12N9/1205 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesize a modified starch. Therefore, the present invention also concerns the starches synthesized from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention.Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加 转基因 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这些类型的植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法以及这些改性淀粉的淀粉衍生物的制备，以及含有本发明淀粉的面粉。 此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有该核酸分子的植物的核酸。 此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

公开(公告)号：US20080276336A1

公开(公告)日：2008-11-06

申请号：US10591419

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： A01H5/00 ,  G01N33/68 ,  C07K14/00 ,  C12Q1/68 ,  C12Q1/25 ,  C07H21/00 ,  C12N5/10

CPC分类号： C12N9/12 ,  C12N15/8245 ,  C12Q1/485 ,  G01N33/573 ,  G01N2400/14 ,  G01N2500/04

摘要： The present invention relates to a method for identifying proteins involved in the phosphorylation of starch and nucleic acids which code for such proteins. The present invention further relates to plant cells and plants which exhibit an altered activity of a protein which can be identified using the method according to the invention. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of this starch and to the manufacture of starch derivatives of this modified starch.

摘要翻译： 本发明涉及一种鉴定涉及淀粉磷酸化的蛋白质的方法以及编码这种蛋白质的核酸。 本发明还涉及使用本发明方法鉴定的蛋白质活性变化的植物细胞和植物。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉以及制备该淀粉的方法和该淀粉衍生物的制造。

公开(公告)号：US08895804B2

公开(公告)日：2014-11-25

申请号：US13174228

申请日：2011-06-30

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/29 ,  C12N15/63 ,  A01H5/00 ,  B02C9/00 ,  C12N9/12

CPC分类号： B02C9/00 ,  C12N9/12 ,  C12N9/1205 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesize a modified starch. Therefore, the present invention also concerns the starches synthesized from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention.Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加 转基因 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这些类型的植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法，以及这些改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉。 此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有该核酸分子的植物的核酸。 此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

公开(公告)号：US07919682B2

公开(公告)日：2011-04-05

申请号：US10591540

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/11 ,  C12N15/63 ,  C07H21/02 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/00 ,  C08B31/066 ,  C12N9/1294 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OK1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention.Furthermore, the present invention relates to nucleic acid molecules, which are suitable for manufacturing plants according to the invention.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性降低，与相应的野生型植物细胞或未经遗传修饰的野生型植物相比较 。 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉 。 此外，本发明涉及适合于制造根据本发明的植物的核酸分子。

公开(公告)号：US20080022421A1

公开(公告)日：2008-01-24

申请号：US10591428

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/02 ,  C08B31/00 ,  C12N15/00 ,  C12N15/82

CPC分类号： B02C9/00 ,  C12N9/12 ,  C12N9/1205 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starches synthesised from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加 转基因 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这些类型的植物细胞和植物合成了改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法以及这些改性淀粉的淀粉衍生物的制备，以及含有本发明淀粉的面粉。 此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有该核酸分子的植物的核酸。 此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

公开(公告)号：US08257502B2

公开(公告)日：2012-09-04

申请号：US13033495

申请日：2011-02-23

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C08B31/06 ,  C08B30/00 ,  C07H11/04 ,  C07K14/415

CPC分类号： C12N9/00 ,  C08B31/066 ,  C12N9/1294 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OK1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules, which are suitable for manufacturing plants according to the invention.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性降低，与相应的野生型植物细胞或未经遗传修饰的野生型植物相比较 。 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉 。 此外，本发明涉及适合于制造根据本发明的植物的核酸分子。

公开(公告)号：US08007592B2

公开(公告)日：2011-08-30

申请号：US12774420

申请日：2010-05-05

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C08B31/06 ,  C08B30/00 ,  C07H11/04 ,  C07K14/415

CPC分类号： B02C9/00 ,  C12N9/12 ,  C12N9/1205 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starches synthesised from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加 转基因 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这些类型的植物细胞和植物合成了改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法以及这些改性淀粉的淀粉衍生物的制备，以及含有本发明淀粉的面粉。 此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有该核酸分子的植物的核酸。 此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

公开(公告)号：US07932436B2

公开(公告)日：2011-04-26

申请号：US10591432

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/29 ,  C12N15/63 ,  A01H5/00

CPC分类号： C12N15/8245 ,  C08B31/066 ,  C12N9/00

摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the increase of the activity of a starch phosphorylating OK1 protein and a starch phosphorylating R1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesize a modified starch. The present invention therefore also relates to the starch synthesized by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention.Furthermore, the present invention relates to nucleic acid molecules and vectors containing sequences which code for an OK1 protein and an R1 protein, as well as host cells which contain these nucleic acid molecules.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中与相应的野生型植物细胞或野生型植物相比，遗传修饰导致淀粉磷酸化OK1蛋白和淀粉磷酸化R1蛋白的活性增加 没有转基因。 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉 。 此外，本发明涉及含有编码OK1蛋白和R1蛋白的序列的核酸分子和载体以及含有这些核酸分子的宿主细胞。

公开(公告)号：US20070163004A1

公开(公告)日：2007-07-12

申请号：US10591540

申请日：2005-03-04

申请人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

发明人： Claus Frohberg ,  Oliver Koetting ,  Gerhard Ritte ,  Martin Steup

IPC分类号： A01H5/00 ,  C08B31/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/00 ,  C08B31/066 ,  C12N9/1294 ,  C12N15/8245

摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OK1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules, which are suitable for manufacturing plants according to the invention.

摘要翻译： 本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性降低，与相应的野生型植物细胞或未经遗传修饰的野生型植物相比较 。 此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。 这种植物细胞和植物合成改性淀粉。 因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉 。 此外，本发明涉及适合于制造根据本发明的植物的核酸分子。