公开(公告)号：US5538864A

公开(公告)日：1996-07-23

申请号：US373877

申请日：1995-01-17

申请人： Colin J. Hill ,  Todd R. Klaenhammer

发明人： Colin J. Hill ,  Todd R. Klaenhammer

IPC分类号： A23C9/123 ,  C12N1/04 ,  C12N1/21 ,  C12N15/11 ,  C12N15/34 ,  C12N15/65 ,  C12N15/74 ,  C12N1/20 ,  A23C9/00 ,  C12P7/56

CPC分类号： C12N15/65 ,  A23C9/1236 ,  C12N1/04 ,  C12N15/11 ,  C12N15/746

摘要： Recombinant bacteria containing phage-encoded resistance ("Per") and methods of making and using the same are disclosed. Such bacteria are made by (a) conducting a fermentation of a substrate in a medium containing a defined bacterial culture until bacteriophage are detected in the medium, the bacteriophage being specific to at least one bacteria in the medium; (b) isolating the bacteriophage; (c) digesting DNA of the bacteriophage to produce a library of DNA fragments; (d) transforming the bacteria susceptible to said bacteriophage with the library of DNA fragments to provide transformed bacteria; (e) selecting from among the transformed bacteria, a bacteriophage-resistant transformed bacteria; (f) adding bacteriophage resistant transformed bacteria to the medium; and (g) recommencing step (a). Also disclosed are bacterial cells which contain a first bacteriophage defense mechanism (Per), wherein Per comprises a bacteriophage origin of replication (ori) operatively associated with a DNA sequence incapable of producing live bacteriophage. The bacterial cell is capable of being infected by a bacteriophage, the DNA of which, once injected into the bacterial cell, competes with Per for binding to DNA polymerase.

摘要翻译： 公开了含有噬菌体编码抗性（“Per”）的重组细菌及其制备和使用方法。 这样的细菌通过以下方法制备：（a）在含有限定的细菌培养基的培养基中进行底物的发酵，直到在培养基中检测到噬菌体，噬菌体对培养基中的至少一种细菌是特异性的; （b）分离噬菌体; （c）消化噬菌体的DNA以产生DNA片段文库; （d）用DNA片段的文库转化对所述噬菌体易感的细菌，以提供转化的细菌; （e）从转化细菌中选择抗噬菌体转化细菌; （f）向培养基中加入抗噬菌体转化细菌; 和（g）重新开始步骤（a）。 还公开了含有第一噬菌体防御机制（Per）的细菌细胞，其中Per包含与不能产生活噬菌体的DNA序列可操作地相关的噬菌体复制起点（ori）。 细菌细胞能够被噬菌体感染，其噬菌体一旦被注入到细菌细胞中就与Per结合就与DNA聚合酶结合。

公开(公告)号：US5593885A

公开(公告)日：1997-01-14

申请号：US375072

申请日：1995-01-18

申请人： Todd R. Klaenhammer ,  Wesley D. Sing ,  Colin J. Hill

发明人： Todd R. Klaenhammer ,  Wesley D. Sing ,  Colin J. Hill

IPC分类号： C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/52 ,  C12N15/74

CPC分类号： C12N15/00 ,  C12N1/20 ,  C12N15/52 ,  C12N15/746

摘要： A phage defense rotation strategy for use in the successive fermentations of a substrate in a fermentation plant is disclosed. The strategy comprises (a) fermenting substrate with a first bacterial culture comprising a bacterial strain capable of fermenting the substrate and, preferably, carrying a first phage defense mechanism; and then (b) fermenting the substrate with a second bacterial culture comprising a second bacterial strain isogenic with the first bacterial strain, wherein the second strain carries a second phage defense mechanism different from the first phage defense mechanism.Also disclosed is a mixed bacterial culture capable of fermenting a substrate. The mixed culture comprises (a) a first bacterial strain carrying a first phage defense mechanism; and (b) a second bacterial strain isogenic with the first strain, wherein the second strain carries a phage defense mechanism different from the phage defense mechanism carried by the first strain.

摘要翻译： 公开了用于发酵设备中底物连续发酵的噬菌体防御旋转策略。 该策略包括：（a）用包含能够发酵底物的细菌菌株的第一细菌培养物发酵底物，优选携带第一噬菌体防御机制; 然后（b）用包含与第一细菌菌株同基因的第二细菌菌株的第二细菌培养物发酵底物，其中第二菌株携带与第一噬菌体防御机制不同的第二噬菌体防御机制。 还公开了能够发酵底物的混合细菌培养物。 混合培养物包含（a）携带第一噬菌体防御机制的第一菌株; 和（b）与第一菌株同基因的第二菌株，其中第二菌株携带不同于第一菌株携带的噬菌体防御机制的噬菌体防御机制。