公开(公告)号：US20070215809A1

公开(公告)日：2007-09-20

申请号：US11568937

申请日：2005-05-05

申请人： Colin Mansfield ,  Anthony Shaw

发明人： Colin Mansfield ,  Anthony Shaw

IPC分类号： G01N21/35

CPC分类号： G01N21/35 ,  G01N21/03 ,  G01N2001/4016 ,  G01N2001/4072

摘要： A number of reagent-free infrared spectroscopic diagnostic and analytical methods have been established previously, making use of dry biofluid films. For example, this approach has successfully measured high concentration analytes of serum and urine. However, a number of low concentration diagnostically relevant analytes presently elude detection by infrared spectroscopy. This is due in part to their relatively low concentration and in part to spectral interference by other strongly absorbing constituents. The applicability of the technique would be broadened substantially if it were possible to separate and concentrate, lower concentration analytes, e.g. serum creatinine and urine proteins, from the obscuring presence of relatively high concentration compounds. The invention disclosed achieves this is through microfluidic sample preconditioning based upon laminar fluid diffusion interfaces. Preferential separation of certain low concentration serum and urine analytes of clinical interest that presently lie just below the threshold of detection by infrared spectroscopy is provided.

摘要翻译： 以前已经建立了许多无试剂的红外光谱诊断和分析方法，利用干生物流体膜。 例如，这种方法已成功测量血清和尿液的高浓度分析物。 然而，许多低浓度诊断相关分析物目前排除通过红外光谱检测。 这部分归因于它们的相对低的浓度，部分是由于其它强吸收组分的光谱干扰。 如果可以分离和浓缩，降低浓度的分析物，例如，可以将该技术的适用性显着扩大。 血清肌酐和尿蛋白，来自相对高浓度化合物的模糊存在。 所公开的发明通过基于层流扩散界面的微流体样品预处理来实现。 提供目前正在低于通过红外光谱法检测阈值的临床兴趣的某些低浓度血清和尿液分析物的优先分离。

公开(公告)号：US07754490B2

公开(公告)日：2010-07-13

申请号：US11568937

申请日：2005-05-05

申请人： Colin Mansfield ,  Anthony Shaw

发明人： Colin Mansfield ,  Anthony Shaw

IPC分类号： G01N21/35 ,  G01N21/17 ,  G01N21/00

CPC分类号： G01N21/35 ,  G01N21/03 ,  G01N2001/4016 ,  G01N2001/4072

摘要： A number of reagent-free infrared spectroscopic diagnostic and analytical methods have been established previously, making use of dry biofluid films. For example, this approach has successfully measured high concentration analytes of serum and urine. However, a number of low concentration diagnostically relevant analytes presently elude detection by infrared spectroscopy. This is due in part to their relatively low concentration and in part to spectral interference by other strongly absorbing constituents. The applicability of the technique would be broadened substantially if it were possible to separate and concentrate, lower concentration analytes, e.g. serum creatinine and urine proteins, from the obscuring presence of relatively high concentration compounds. The invention disclosed achieves this is through microfluidic sample preconditioning based upon laminar fluid diffusion interfaces. Preferential separation of certain low concentration serum and urine analytes of clinical interest that presently lie just below the threshold of detection by infrared spectroscopy is provided.

摘要翻译： 以前已经建立了许多无试剂的红外光谱诊断和分析方法，利用干生物流体膜。 例如，这种方法已成功测量血清和尿液的高浓度分析物。 然而，许多低浓度诊断相关分析物目前排除通过红外光谱检测。 这部分归因于它们的相对低的浓度，部分是由于其它强吸收组分的光谱干扰。 如果可以分离和浓缩，降低浓度的分析物，例如，可以将该技术的适用性显着扩大。 血清肌酐和尿蛋白，来自相对高浓度化合物的模糊存在。 所公开的发明通过基于层流扩散界面的微流体样品预处理来实现。 提供目前正在低于通过红外光谱法检测阈值的临床兴趣的某些低浓度血清和尿液分析物的优先分离。