公开(公告)号：US09803239B2

公开(公告)日：2017-10-31

申请号：US13840482

申请日：2013-03-15

Applicant: Complete Genomics, Inc.

Inventor： Bill J. Peck ,  Mark Fuller ,  Daniel West ,  Anthony Delacruz

IPC: B01L3/00 ,  C12Q1/68 ,  G01N21/05

CPC classification number: C12Q1/6874 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/565 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  G01N21/05

Abstract: Biochemical flow cells having sealed inlets and outlets are provided for performing high-volume assays on macromolecules. In one example embodiment, a flow cell with detachable inlet and outlet connectors comprises an inlet manifold, a coverslip, and a substrate disposed below the coverslip to form a reaction chamber, where the substrate is disposed to partially cover the inlet manifold such that a slit is formed along an entire edge of the substrate where fluids can flow from the inlet manifold through the slit, around substantially the entire edge of the substrate, and into the reaction chamber at equalized pressure and without bubbles. In another embodiment, a flow cell comprises an outlet manifold, two or more flow regions each connected to its own loading port via its own flow distribution funnel, each loading port connected to the outlet manifold, and plugs in a wall of the outlet manifold opposite each loading port, such that when a plug is absent from the wall of the outlet manifold, a loading tip may be inserted in its place, passing through the outlet manifold and connecting directly to a loading port.

公开(公告)号：US20130281305A1

公开(公告)日：2013-10-24

申请号：US13840482

申请日：2013-03-15

Applicant: COMPLETE GENOMICS, INC.

Inventor： Bill J. Peck ,  Mark Fuller ,  Daniel West ,  Anthony Delacruz

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/565 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  G01N21/05

Abstract: Biochemical flow cells having sealed inlets and outlets are provided for performing high-volume assays on macromolecules. In one example embodiment, a flow cell with detachable inlet and outlet connectors comprises an inlet manifold, a coverslip, and a substrate disposed below the coverslip to form a reaction chamber, where the substrate is disposed to partially cover the inlet manifold such that a slit is formed along an entire edge of the substrate where fluids can flow from the inlet manifold through the slit, around substantially the entire edge of the substrate, and into the reaction chamber at equalized pressure and without bubbles. In another embodiment, a flow cell comprises an outlet manifold, two or more flow regions each connected to its own loading port via its own flow distribution funnel, each loading port connected to the outlet manifold, and plugs in a wall of the outlet manifold opposite each loading port, such that when a plug is absent from the wall of the outlet manifold, a loading tip may be inserted in its place, passing through the outlet manifold and connecting directly to a loading port.

Abstract translation: 提供具有密封入口和出口的生化流动池用于在大分子上进行高体积测定。 在一个示例性实施例中，具有可拆卸入口和出口连接器的流动池包括入口歧管，盖玻片和布置在盖玻片下方的基底，以形成反应室，其中基底设置成部分地覆盖入口歧管， 沿着衬底的整个边缘形成，其中流体可以从入口歧管流过狭缝，在基本上基本上整个边缘周围流动，并以均压和无气泡进入反应室。 在另一个实施例中，流动池包括出口歧管，两个或更多个流动区域，每个流动区域通过其自身的流量分配漏斗连接到其自己的装载端口，每个装载端口连接到出口歧管，并且插塞在出口歧管的壁上相对 每个装载端口，使得当插座从出口歧管的壁不存在时，装载尖端可插入其位置，通过出口歧管并直接连接到装载端口。