公开(公告)号：US20180002735A1

公开(公告)日：2018-01-04

申请号：US15457471

申请日：2017-03-13

Applicant: Complete Genomics, Inc.

Inventor： Radoje T. Drmanac ,  Matthew J. Callow

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2539/103 ,  C12Q2531/119

Abstract: The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.

公开(公告)号：US20160378916A1

公开(公告)日：2016-12-29

申请号：US15195741

申请日：2016-06-28

Applicant: Complete Genomics, Inc.

Inventor： Radoje T. Drmanac ,  Brock A. Peters ,  Bahram Ghaffarzadeh Kermani

IPC: G06F19/22 ,  C12Q1/68

CPC classification number: G16B30/00 ,  C12Q1/6869 ,  C12Q2537/159 ,  C12Q2549/10

Abstract: The present invention is directed to logic for analysis of nucleic acid sequence data that employs algorithms that lead to a substantial improvement in sequence accuracy and that can be used to phase sequence variations, e.g., in connection with the use of the long fragment read (LFR) process.

Abstract translation: 本发明涉及用于分析核酸序列数据的逻辑，其使用导致序列精度的实质性改进并且可用于相序变化的算法，例如结合使用长片段读取（LFR ）过程。

公开(公告)号：US20160194686A1

公开(公告)日：2016-07-07

申请号：US14961753

申请日：2015-12-07

Applicant: Complete Genomics, Inc.

Inventor： Radoje T. Drmanac ,  Matthew J. Callow

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2539/103 ,  C12Q2531/119

Abstract: The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.

Abstract translation: 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。

公开(公告)号：US09238834B2

公开(公告)日：2016-01-19

申请号：US13647338

申请日：2012-10-08

Applicant: Complete Genomics, Inc.

Inventor： Radoje T. Drmanac ,  Clifford Reid

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2549/119 ,  C12Q2537/149 ,  C12Q2565/137

Abstract: Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure.

Abstract translation: 提供了有效的霰弹枪测序的方法，以允许文库中包含的感兴趣的核酸的有效选择和测序。 感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。 感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。 感兴趣的其他核酸包括基因组DNA，RNA或cDNAs（外显子，基因，基因组，转录本）的子集。 通过设计一种高效（简单易于实施，快速，高特异性，低成本）选择程序，通过在初始测序程序中使用高度冗余的霰弹枪测序，以更少的努力实现更完整的顺序。