公开(公告)号：US20050070695A1

公开(公告)日：2005-03-31

申请号：US10785673

申请日：2004-02-23

申请人： Conceicao Minetti ,  Francis Michon ,  Jeffrey Pullen ,  Mary Polvino-Bodnar ,  Shu-Mei Liang ,  Joseph Tai

发明人： Conceicao Minetti ,  Francis Michon ,  Jeffrey Pullen ,  Mary Polvino-Bodnar ,  Shu-Mei Liang ,  Joseph Tai

IPC分类号： C12N15/09 ,  C07K14/195 ,  C07K14/315 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C07K14/47

CPC分类号： C07K14/3156 ,  A61K39/092 ,  A61K2039/6037 ,  Y10S424/831 ,  Y10S530/825

摘要： This invention relates to modified pneumolysin polypeptides that retain the immunogenic nature of pneumolysin but have reduced or undetectable hemolytic activity compared to native pneumolysin. The invention also provides a method for generating novel pneumolysin variants with these desired characteristic properties. The invention also provides immunogenic compositions useful as pharmaceutical compositions including vaccines in which non-toxic, modified pneumolysin is used to stimulate protective immunity against Streptococcus pneumoniae. The vaccines may be compositions in which the modified pneumolysin is conjugated to bacterial polysaccharides or may be carried on an attenuated viral vector. In addition, the invention also provides a method of using the non-toxic, modified pneumolysin toxoid in order to stimulate antibodies against Streptococcus pneumoniae in a treated individual which are then isolated and transferred to a second individual, thereby conferring protection against Streptococcus pneumoniae in the second individual.

摘要翻译： 本发明涉及与天然气溶素相比，保留免疫原性的气溶菌素的修饰的气溶胶素多肽，但具有降低的或不可检测的溶血活性。 本发明还提供了一种产生具有这些所需特征性质的新型肺炎球菌变异体的方法。 本发明还提供可用作药物组合物的免疫原性组合物，包括其中无毒的改良的肺炎溶血杆菌刺激对肺炎链球菌的保护性免疫的疫苗。 疫苗可以是其中改性气溶胶与细菌多糖缀合或可以携带在减毒病毒载体上的组合物。 此外，本发明还提供了一种使用无毒的改良型肺炎杆菌素类毒素以刺激处理的个体中针对肺炎链球菌的抗体的方法，然后将其分离并转移到第二个体中，从而在肺炎链球菌中得到保护 第二个人。