公开(公告)号：US09512434B2

公开(公告)日：2016-12-06

申请号：US14630308

申请日：2015-02-24

申请人： Cornell Research Foundation, Inc.

发明人： Matthew Delisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

IPC分类号： C12N15/70 ,  C12N9/10 ,  C12N9/14

CPC分类号： C12N15/70 ,  C07K16/1278 ,  C07K2317/21 ,  C07K2317/41 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/14 ,  C12P21/005 ,  C12Y204/01132 ,  C12Y204/01142 ,  C12Y204/01255 ,  C12Y204/01257 ,  C12Y306/03001

摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

摘要翻译： 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核的甘露糖基转移酶活性。 还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。 本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法 衍生自细菌，并以高通量格式分析标签。 还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。