公开(公告)号：US20150165013A1

公开(公告)日：2015-06-18

申请号：US14411770

申请日：2013-07-01

申请人： Lysando AG

发明人： Stefan Miller

IPC分类号： A61K39/04 ,  C12N1/20 ,  C12N1/06 ,  C07K16/18

CPC分类号： A61K39/04 ,  A61K35/74 ,  C07K16/18 ,  C07K2319/55 ,  C12N1/06 ,  C12N1/20 ,  C12N9/1048 ,  C12N9/2462 ,  C12N9/50 ,  C12Y204/01255 ,  C12Y302/01017

摘要： The present invention relates to a method for the preparation of a mycobacterial lysate comprising the steps of: a) contacting a sample comprising at least one Mycobacterium species with a composition having the activity of degrading the cell wall of a Mycobacterium species, the composition comprising: (a) a first fusion protein including (i) a first endolysin or a first domain, both having a first enzymatic activity, the enzymatic activity being at least one or more of the following: N-acetyl-b-D-muramidase (lysozyme, lytic transglycosylase), N-acetyl-b-D-glucosaminidase, N-acetylmuramoyl-L-alanine amidase, L-alanoyl-D-glutamate (LD) endopeptidase, c-D-glutamyl-meso-diaminopimelic acid (DL) peptidase, L-alanyl-D-iso-glutaminyl-meso-diaminopimelic acid (D-Ala-m-DAP) (DD) endopeptidase, or m-DAP-m-DAP (LD) endopeptidase; and (ii) at least one peptide stretch fused to the N- or C-terminus of the endolysin having the first enzymatic activity or the domain having the first enzymatic activity, wherein the peptide stretch is selected from the group consisting of synthetic amphipathic peptide, synthetic cationic peptide, synthetic polycationic peptide, synthetic hydrophobic peptide, synthetic antimicrobial peptide (AMP) or naturally occurring AMP; and (b) a second fusion protein including (i) a second endolysin or a second domain, both having a second enzymatic activity, the enzymatic activity being at least one or more of the following: lipolytic activity, cutinase, mycolarabinogalactanesterase, or alpha/beta hydrolase; and (ii) at least one peptide stretch fused to the N- or C-terminus of the endolysin having a second enzymatic activity or the domain having the second enzymatic activity, wherein the peptide stretch is selected from the group consisting of synthetic amphipathic peptide, synthetic cationic peptide, synthetic polycationic peptide, synthetic hydrophobic peptide, synthetic antimicrobial peptide (AMP) or naturally occurring AMP; b) incubating the sample for a distinct period, and c) isolating the mycobacterial lysate resulting from step b) thereby obtaining the mycobacterial lysate. Moreover, the present invention relates to the a mycobacterial lysate obtained by the method of the present invention and further to a vaccine composition comprising the mycobacterial lysate, an antibody or antibody fragment generated with the mycobacterial lysate or the vaccine, and a pharmaceutical composition comprising the antibody or antibody fragment.

公开(公告)号：US20130171692A1

公开(公告)日：2013-07-04

申请号：US13409558

申请日：2012-03-01

申请人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroke Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12N1/19 ,  C12P21/00

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。

公开(公告)号：US09512434B2

公开(公告)日：2016-12-06

申请号：US14630308

申请日：2015-02-24

申请人： Cornell Research Foundation, Inc.

发明人： Matthew Delisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

IPC分类号： C12N15/70 ,  C12N9/10 ,  C12N9/14

CPC分类号： C12N15/70 ,  C07K16/1278 ,  C07K2317/21 ,  C07K2317/41 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/14 ,  C12P21/005 ,  C12Y204/01132 ,  C12Y204/01142 ,  C12Y204/01255 ,  C12Y204/01257 ,  C12Y306/03001

摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

摘要翻译： 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核的甘露糖基转移酶活性。 还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。 本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法 衍生自细菌，并以高通量格式分析标签。 还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。

公开(公告)号：US08999668B2

公开(公告)日：2015-04-07

申请号：US12811788

申请日：2009-01-05

申请人： Matthew DeLisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

发明人： Matthew DeLisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

IPC分类号： C40B30/06 ,  C12N1/00 ,  C12N9/14 ,  C07K16/12 ,  C12N9/10 ,  C12P21/00

CPC分类号： C12N15/70 ,  C07K16/1278 ,  C07K2317/21 ,  C07K2317/41 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/14 ,  C12P21/005 ,  C12Y204/01132 ,  C12Y204/01142 ,  C12Y204/01255 ,  C12Y204/01257 ,  C12Y306/03001

摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

摘要翻译： 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核甘露糖基转移酶活性。 还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。 本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法 衍生自细菌，并以高通量格式分析标签。 还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。

公开(公告)号：US20230212588A1

公开(公告)日：2023-07-06

申请号：US17806138

申请日：2022-06-09

申请人： Bio Capital Holdings, LLC

发明人： Raul CUERO RENGIFO

IPC分类号： C12N15/52 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/82

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/1022 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/1205 ,  C12N15/8243 ,  C12Y202/01007 ,  C12Y204/01255 ,  C12Y205/01029 ,  C12Y207/01001 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y503/03002

摘要： Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing additives to food, pharmaceutical, and nutritional supplement products.

公开(公告)号：US20170166897A1

公开(公告)日：2017-06-15

申请号：US14774800

申请日：2014-03-11

申请人： UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

发明人： David Rubenstein ,  Kasper Runager

IPC分类号： C12N15/113 ,  A61K45/06 ,  A61K31/7088

CPC分类号： C12N15/1137 ,  A61K31/7088 ,  A61K45/06 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12Y204/01186 ,  C12Y204/01255

摘要： The presently disclosed subject matter provides compounds, compositions, and methods for targeting UDP-N-acetylglucosamine polypeptide β-N-acetylglucosaminyl transferase (OGT). Further, the presently disclosed subject matter provides compounds, compositions, and methods for promoting wound healing.

公开(公告)号：US20150232861A1

公开(公告)日：2015-08-20

申请号：US14630308

申请日：2015-02-24

申请人： CORNELL RESEARCH FOUNDATION, INC.

发明人： Matthew DELISA ,  Cassandra GUARINO ,  Thomas MANSELL ,  Adam FISHER

IPC分类号： C12N15/70 ,  C12N9/14 ,  C12N9/10

CPC分类号： C12N15/70 ,  C07K16/1278 ,  C07K2317/21 ,  C07K2317/41 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/14 ,  C12P21/005 ,  C12Y204/01132 ,  C12Y204/01142 ,  C12Y204/01255 ,  C12Y204/01257 ,  C12Y306/03001

摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

摘要翻译： 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核的甘露糖基转移酶活性。 还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。 本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法 衍生自细菌，并以高通量格式分析标签。 还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。

公开(公告)号：US09017969B2

公开(公告)日：2015-04-28

申请号：US13409558

申请日：2012-03-01

申请人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

发明人： Hiroko Abe ,  Kazuya Tomimoto ,  Yasuko Fujita ,  Tomoko Iwaki ,  Yasunori Chiba ,  Yoshihiro Nakajima ,  Kenichi Nakayama ,  Masatoshi Kataoka ,  Shouki Yatsushiro ,  Shohei Yamamura

IPC分类号： C12P21/00 ,  C12N1/19 ,  C12N1/16 ,  C12N9/10 ,  C12N9/34 ,  C12N9/60 ,  C07K14/47 ,  C12N9/24 ,  C12N15/09

CPC分类号： C12P21/005 ,  C07K14/4726 ,  C07K2319/03 ,  C12N1/16 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2428 ,  C12N9/2488 ,  C12N9/60 ,  C12N15/09 ,  C12Y204/01109 ,  C12Y204/01132 ,  C12Y204/01255 ,  C12Y302/01003 ,  C12Y302/01113 ,  C12Y304/21048 ,  C12Y304/23025

摘要： The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.

摘要翻译： 本发明提供：遗传修饰的酵母，例如具有产生Man5GlcNAc2的N-连接糖链的能力的突变型酵母和产生O-连接的糖链的能力降低的突变体酵母，具有产生Man5GlcNAc2的N-连接糖链的能力的突变体酵母 并且还具有产生GlcNAc1Man5GlcNAc2的N-连接的糖链的能力，以及具有增强的产生和分泌蛋白质的能力和产生Man5GlcNAc2的N-连接的糖链的能力的突变体酵母; 以及使用它们生产糖蛋白的方法。

公开(公告)号：US20110039729A1

公开(公告)日：2011-02-17

申请号：US12811788

申请日：2009-01-05

申请人： Matthew Delisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

发明人： Matthew Delisa ,  Cassandra Guarino ,  Thomas Mansell ,  Adam Fisher

IPC分类号： C40B30/06 ,  C12N1/00 ,  C07K14/00 ,  C12P21/00 ,  C07K16/00

CPC分类号： C12N15/70 ,  C07K16/1278 ,  C07K2317/21 ,  C07K2317/41 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/14 ,  C12P21/005 ,  C12Y204/01132 ,  C12Y204/01142 ,  C12Y204/01255 ,  C12Y204/01257 ,  C12Y306/03001

摘要： The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

摘要翻译： 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中真核糖基转移酶活性是真核的二酰基连接的UDP-GlcNAc转移酶活性和真核甘露糖基转移酶活性。 还公开了通过提供包含真核糖基转移酶活性的原核宿主细胞并在有效产生糖基化蛋白的条件下培养原核宿主细胞来生产糖基化蛋白的方法。 本发明的另一方面涉及通过在细菌表面上表达一种或多种聚糖以在细菌表面上或噬菌体的表面上附着一个或多个聚糖上的标记物来筛选细菌或噬菌体的方法 衍生自细菌，并以高通量格式分析标签。 还公开了包含识别和结合天然抗原的Fv部分的糖基化抗体和在保守的天冬酰胺残基处被糖基化的Fc部分。