公开(公告)号：US10319502B2

公开(公告)日：2019-06-11

申请号：US15520581

申请日：2015-10-23

申请人： CORNING INCORPORATED

发明人： Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC分类号： H01F1/12 ,  H01F1/00 ,  C12N15/10 ,  H01F1/34 ,  H01F1/36 ,  H01F1/20

摘要： Magnetic particles (100) have a particle size (134) of 500 nm or less and include a core (110) and a polymer coating (120) that surrounds and encapsulates the core (110). The core (110) includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Yb, Dy, Gd or Er and Nb. The magnetic core (100) is a polycrystalline particle and is a superspin glass magnetic material, having a coercivity greater than zero and a magnetic remenance greater than zero at room temperature. Above room temperature and at low field, the magnetic moment of these superspin glass magnetic materials increases with temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles (100).

公开(公告)号：US11667906B2

公开(公告)日：2023-06-06

申请号：US14950771

申请日：2015-11-24

申请人： Corning Incorporated

发明人： Stephen Joseph Caracci ,  Ye Fang ,  Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC分类号： C12N13/00 ,  C12N1/02 ,  H01F1/37 ,  H01F1/26

CPC分类号： C12N13/00 ,  C12N1/02 ,  H01F1/26 ,  H01F1/37

摘要： Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.

公开(公告)号：US20190326039A1

公开(公告)日：2019-10-24

申请号：US16391866

申请日：2019-04-23

申请人： Corning Incorporated

发明人： Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC分类号： H01F1/12 ,  H01F1/00 ,  C12N15/10

摘要： Magnetic particles have a particle size of 500 nm of less and include a core and a polymer coating that surrounds and encapsulates the core. The core includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Ln, Yb, Dy, Gd or Er and Nb. The magnetic core is polycrystalline particles which are superspin glass magnetic materials having coercivity greater than zero and magnetic remenance greater than zero at room temperature. Magnetic moment of these superspin glass magnetic materials at low field show increasing with temperature over room temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles.

公开(公告)号：US20180151278A1

公开(公告)日：2018-05-31

申请号：US15520581

申请日：2015-10-23

申请人： CORNING INCORPORATED

发明人： Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC分类号： H01F1/12 ,  H01F1/00 ,  C12N15/10 ,  H01F1/34 ,  H01F1/36 ,  H01F1/20

CPC分类号： H01F1/12 ,  C12N15/1013 ,  H01F1/0054 ,  H01F1/20 ,  H01F1/344 ,  H01F1/36

摘要： Magnetic particles (100) have a particle size (134) of 500 nm or less and include a core (110) and a polymer coating (120) that surrounds and encapsulates the core (110). The core (110) includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Yb, Dy, Gd or Er and Nb. The magnetic core (100) is a polycrystalline particle and is a superspin glass magnetic material, having a coercivity greater than zero and a magnetic remenance greater than zero at room temperature. Above room temperature and at low field, the magnetic moment of these superspin glass magnetic materials increases with temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles (100).

公开(公告)号：US20160145600A1

公开(公告)日：2016-05-26

申请号：US14950771

申请日：2015-11-24

申请人： Corning Incorporated

发明人： Stephen Joseph Caracci ,  Ye Fang ,  Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC分类号： C12N13/00 ,  H01F1/147 ,  H01F1/20 ,  C12N5/00 ,  G01N1/40

CPC分类号： C12N13/00 ,  C12N1/02 ,  H01F1/26 ,  H01F1/37

摘要： Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.

摘要翻译： 磁性微载体珠具有1至1000微米的粒度，并且包括复合芯和围绕并封装芯的聚合物涂层。 复合芯包括嵌入在难消化的聚合物基质内的磁性颗粒。 该涂层是可消化或消化不良的聚合物，其在细胞培养期间促进细胞与珠表面的粘附。 可以使用磁力在细胞培养过程中搅动微载体珠，以及将珠与培养的细胞或加工的生物培养基分离。