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公开(公告)号：US11111470B2
公开(公告)日：2021-09-07
申请号：US17039218
申请日：2020-09-30
申请人： CORNING INCORPORATED
摘要： A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.
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公开(公告)号：US20190326039A1
公开(公告)日：2019-10-24
申请号：US16391866
申请日：2019-04-23
申请人： Corning Incorporated
发明人： Ann MeeJin Ferrie , Yan Jin , Lingyan Wang , Yue Zhou
摘要： Magnetic particles have a particle size of 500 nm of less and include a core and a polymer coating that surrounds and encapsulates the core. The core includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Ln, Yb, Dy, Gd or Er and Nb. The magnetic core is polycrystalline particles which are superspin glass magnetic materials having coercivity greater than zero and magnetic remenance greater than zero at room temperature. Magnetic moment of these superspin glass magnetic materials at low field show increasing with temperature over room temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles.
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公开(公告)号：US20180151278A1
公开(公告)日：2018-05-31
申请号：US15520581
申请日：2015-10-23
申请人： CORNING INCORPORATED
发明人： Ann MeeJin Ferrie , Yan Jin , Lingyan Wang , Yue Zhou
CPC分类号： H01F1/12 , C12N15/1013 , H01F1/0054 , H01F1/20 , H01F1/344 , H01F1/36
摘要： Magnetic particles (100) have a particle size (134) of 500 nm or less and include a core (110) and a polymer coating (120) that surrounds and encapsulates the core (110). The core (110) includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Yb, Dy, Gd or Er and Nb. The magnetic core (100) is a polycrystalline particle and is a superspin glass magnetic material, having a coercivity greater than zero and a magnetic remenance greater than zero at room temperature. Above room temperature and at low field, the magnetic moment of these superspin glass magnetic materials increases with temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles (100).
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公开(公告)号：US20160145600A1
公开(公告)日：2016-05-26
申请号：US14950771
申请日：2015-11-24
申请人： Corning Incorporated
发明人： Stephen Joseph Caracci , Ye Fang , Ann MeeJin Ferrie , Yan Jin , Lingyan Wang , Yue Zhou
摘要： Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.
摘要翻译： 磁性微载体珠具有1至1000微米的粒度，并且包括复合芯和围绕并封装芯的聚合物涂层。 复合芯包括嵌入在难消化的聚合物基质内的磁性颗粒。 该涂层是可消化或消化不良的聚合物，其在细胞培养期间促进细胞与珠表面的粘附。 可以使用磁力在细胞培养过程中搅动微载体珠，以及将珠与培养的细胞或加工的生物培养基分离。
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5.发明申请METHOD FOR FORMING A SCRATCH RESISTANT CRYSTALLIZED LAYER ON A SUBSTRATE AND ARTICLE FORMED THEREFROM 有权在基板上形成耐蚀刻结晶层的方法及其形成的制品公开(公告)号：US20160083863A1
公开(公告)日：2016-03-24
申请号：US14853191
申请日：2015-09-14
申请人： CORNING INCORPORATED
CPC分类号： C30B13/00 , C03C17/25 , C03C2217/214 , C03C2218/113 , C03C2218/116 , C03C2218/32 , C23C18/1216 , C23C18/1241 , C23C18/1245 , C23C18/1254 , C23C18/1295 , C30B29/20
摘要： A crystallized layer can be formed on a substrate from a precursor layer deposited on a surface of the substrate. The precursor layer can be an oxide, a nitride, a carbide, or an oxynitride. The process for forming the crystallized layer includes melting the precursor layer formed on the surface of the substrate by localized topical heating of the precursor layer and then cooling the melted precursor layer so that it crystallized to form a scratch resistant crystallized layer. The scratch resistant crystallized layer can have a hardness of 15 GPa or greater.
摘要翻译： 结晶层可以从沉积在基板的表面上的前体层形成在基板上。 前体层可以是氧化物，氮化物，碳化物或氧氮化物。 形成结晶层的方法包括通过局部局部加热前体层熔化形成在基材表面上的前体层，然后冷却熔融的前体层，使其结晶形成耐划伤的结晶层。 耐刮擦结晶层的硬度可以为15GPa以上。
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公开(公告)号：US20220372421A1
公开(公告)日：2022-11-24
申请号：US17766045
申请日：2020-09-29
申请人： CORNING INCORPORATED
摘要： Kits for making a spheroid-stabilizing hydrogel in a calcium-free or calcium-chelated cell culture media include (a) a gelation agent including a polygalacturonic acid (PGA) compound or an alginic acid compound, wherein the PGA compound includes at least one of: (i) pectic acid or salts thereof, or (ii) partially esterified pectic acid having a degree of esterification from about 1 to about 40 mol % or salts thereof; (b) a crosslinking agent, wherein the crosslinking agent includes a salt of a divalent ion; and (c) a proton donor, wherein the proton donor includes lactones, esters, or other compounds that hydrolyze in aqueous solutions to form acids over a period of from 10 minutes to 1 hour. Resultant spheroid-stabilizing hydrogels and methods of preparing the same.
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7.发明申请公开(公告)号：US20200248124A1
公开(公告)日：2020-08-06
申请号：US16781883
申请日：2020-02-04
申请人： CORNING INCORPORATED
摘要： A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
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公开(公告)号：US20200248122A1
公开(公告)日：2020-08-06
申请号：US16781764
申请日：2020-02-04
申请人： CORNING INCORPORATED
摘要： A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
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公开(公告)号：US20200148989A1
公开(公告)日：2020-05-14
申请号：US16628309
申请日：2018-07-13
申请人： CORNING INCORPORATED
摘要： A cell culture vessel (100) has walls and a substrate having a plurality of microcavities (120), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange (170) surrounds the substrate having an array of microcavities. A channel (175, 176) surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.
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公开(公告)号：US20170342363A1
公开(公告)日：2017-11-30
申请号：US15492690
申请日：2017-04-20
申请人： Corning Incorporated
发明人： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Gregory Roger Martin , Kaitlyn Mary Matias , Michael Kurt Schaefer , Allison Jean Tanner
摘要： The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.
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