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1.发明授权Isolated nucleic acid molecule encoding a variant human eukaryotic Intiation Factor 4E protein 有权编码变体人真核起始因子4E蛋白的分离的核酸分子公开(公告)号：US06596854B2
公开(公告)日：2003-07-22
申请号：US09855747
申请日：2001-04-14
IPC分类号： C12N1512
CPC分类号： C07K14/4705
摘要： The invention provides a method of purifying human eIF-4E protein and amino acid sequence variants thereof having altered binding affinity for capped RNA. Using the described purification, amino acid sequence variants can readily be expressed, purified and tested. Both lowered and enhanced binding affinity variants are useful for modifying protein expression levels in vivo and in vitro.
摘要翻译： 本发明提供了纯化具有改变的对封端RNA的结合亲和力的人eIF-4E蛋白质及其氨基酸序列变体的方法。 使用所述纯化，氨基酸序列变体可以容易地进行表达，纯化和测试。 降低和增强的结合亲和力变体都可用于体内和体外修饰蛋白质表达水平。
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2.发明授权Purified eukaryotic-initiation factor 4E having altered RNA binding affinity 失效具有改变的RNA结合亲和力的纯化的真核起始因子4E公开(公告)号：US06232442B1
公开(公告)日：2001-05-15
申请号：US09465615
申请日：1999-12-17
IPC分类号： C07K1447
CPC分类号： C07K14/4705
摘要： The invention provides a method of purifying human eIF-4E protein and amino acid sequence variants thereof having altered binding affinity for capped RNA. Using the described purification, amino acid sequence variants can readily be expressed, purified and tested. Both lowered and enhanced binding affinity variants are useful for modifying protein expression levels in vivo and in vitro.
摘要翻译： 本发明提供了纯化具有改变的对封端RNA的结合亲和力的人eIF-4E蛋白质及其氨基酸序列变体的方法。 使用所述纯化，氨基酸序列变体可以容易地进行表达，纯化和测试。 降低和增强的结合亲和力变体都可用于体内和体外修饰蛋白质表达水平。
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公开(公告)号：US07135320B2
公开(公告)日：2006-11-14
申请号：US09731660
申请日：2000-12-08
CPC分类号： C07K14/4705 , C07K2319/00
摘要： The present invention relates generally to a newly identified adaptor protein FRS2 and related products and methods. FRS2 links protein kinases to activating partners in cells. The invention also relates to nucleic acid molecules encoding portions of FRS2, nucleic acid vectors containing FRS2 related nucleic acid molecules, recombinant cells containing such nucleic acid vectors, polypeptides purified from such recombinant cells, antibodies to such polypeptides, and methods of identifying compounds that enhance or block FRS2 interactions with natural binding partners. Also disclosed are methods for diagnosing abnormal conditions in an organism with FRS2 related molecules or compounds.
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公开(公告)号：US06310181B1
公开(公告)日：2001-10-30
申请号：US08980523
申请日：1997-12-01
IPC分类号： C07K1400
CPC分类号： C07K14/4705 , C07K2319/00
摘要： The present invention relates generally to a newly identified adaptor protein FRS2 and related products and methods. FRS2 links protein kinases to activating partners in cells. The invention also relates to nucleic acid molecules encoding portions of FRS2, nucleic acid vectors containing FRS2 related nucleic acid molecules, recombinant cells containing such nucleic acid vectors, polypeptides purified from such recombinant cells, antibodies to such polypeptides, and methods of identifying compounds that enhance or block FRS2 interactions with natural binding partners. Also disclosed are methods for diagnosing abnormal conditions in an organism with FRS2 related molecules or compounds.
摘要翻译： 本发明一般涉及新鉴定的接头蛋白FRS2及其相关产品和方法。 FRS2将蛋白激酶与细胞中的激活配偶体连接。 本发明还涉及编码部分FRS2的核酸分子，含有FRS2相关核酸分子的核酸载体，含有此类核酸载体的重组细胞，从这些重组细胞纯化的多肽，对这些多肽的抗体，以及鉴定增强的化合物的方法 或阻断与天然结合配偶体的FRS2相互作用。 还公开了用于诊断具有FRS2相关分子或化合物的生物体中的异常状况的方法。
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