公开(公告)号：US20240228530A1

公开(公告)日：2024-07-11

申请号：US18562092

申请日：2022-06-22

Applicant: Cytiva BioProcess R&D AB

Inventor： Bengt Westerlund ,  Mats Ander

IPC: C07K1/22

CPC classification number: C07K1/22 ,  C07K2317/31 ,  C07K2317/56

Abstract: The present invention relates to a method of separating bispecific antibodies or bispecific antibody fragments. The method comprises the steps of a) providing a feed comprising bispecific antibodies or bispecific antibody fragments; b) contacting the feed with a separation matrix having affinity ligands coupled to a support; c) optionally washing the separation resin with a washing liquid; d) applying an elution buffer to the separation resin, to elute the antibodies or antibody fragments bound to the affinity ligand; wherein in step d) a pH gradient is applied over the elution buffer, said pH gradient being from about 6 to about 2.

公开(公告)号：US20210017223A1

公开(公告)日：2021-01-21

申请号：US16981716

申请日：2019-03-26

Applicant: Cytiva BioProcess R&D AB

Inventor： Tomas Bjorkman ,  Bengt Westerlund

IPC: C07K1/36 ,  C07K1/22 ,  C07K1/18 ,  C07K16/00

Abstract: The invention relates to a method of separating immunoglobulin variants, comprising the steps of: a) providing a column packed with an Fc-binding affinity chromatography resin; b) loading a sample comprising at least two Fc-comprising immunoglobulin variants onto the column; c) optionally washing the column with a washing liquid; and d) conveying an eluent through said column to elute at least a target immunoglobulin variant from said column and recovering one or more eluate fractions comprising the target immunoglobulin variant in enriched form.