公开(公告)号：US11352388B2

公开(公告)日：2022-06-07

申请号：US15564224

申请日：2016-04-04

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Mikael Berg ,  Ida Eklind ,  Sandeep Kristiansson

IPC: C07K1/18 ,  B01D15/36 ,  C07K14/755 ,  A61K38/37 ,  A61P7/00 ,  A61K38/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of Factor VIII and von Willebrand factor from a cryoprecipitate of plasma. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US20250066757A1

公开(公告)日：2025-02-27

申请号：US18719120

申请日：2023-02-20

Applicant: Cytiva BioProcess R&D AB

Inventor： Simon Aberg ,  Linda Hagman ,  Mikael Berg ,  Hans Blom ,  Albin Larsson ,  Joakim Bagge ,  Elin Monie ,  Markus Pitkanen

IPC: C12N15/10

Abstract: The present disclosure is directed to a method for separating supercoiled plasmid DNA (pDNA) from a liquid sample. the method comprising the steps of: (a) adding a liquid sample comprising pDNA to a first chromatography material comprising (i) an anion exchange chromatography ligand for binding to pDNA and (ii) a support material allowing convective flow through the first chromatography material, wherein the liquid sample originates from a cell culture harvest and has been subjected to a step of removing RNA before step (a): (b) eluting a liquid sample. comprising a purified mixture of supercoiled pDNA and open circular pDNA, from the first chromatography material: (c) adding the liquid sample from step (b) to a second chromatography material comprising a ligand that binds to pDNA and enables selective separation of supercoiled pDNA from open circular pDNA: (d) eluting the purified supercoiled pDNA from the second chromatography material: wherein the supercoiled pDNA eluted in step (d) has a purity degree of at least 95% without use of any further chromatography material than said first and second chromatography materials. Steps (a)-(d) and any intermediate steps can be completed within 5 hours. Further disclosed are uses of supercoiled pDNA obtained by said separation method.