公开(公告)号：US20220280886A1

公开(公告)日：2022-09-08

申请号：US17619204

申请日：2020-06-16

Applicant: Cytiva Sweden AB

Inventor： Gunnar Malmquist ,  Ola Lind ,  Matthew Townsend ,  Ian Scanlon ,  Zachary Michael Sexton

IPC: B01D15/18 ,  G01N30/86

Abstract: The present invention relates to a method for monitoring operational status in a column capture chromatography system configured for cyclical repetitive purification performed on a volume of sample feed comprising at least one product configured to be captured in the column during loading. The method comprises: performing (51) a purification cycle; measuring (52) at least one parameter during the purification cycle indicative of breakthrough of the at least one captured product after the column during loading of sample feed; when breakthrough is detected, reducing (56a) the amount of sample feed loaded during loading in the next purification cycle; and repeating the steps to perform another purification cycle. Each purification cycle comprising: loading an amount of sample feed onto the column, washing the column and eluting the at least one product.

公开(公告)号：US20220111309A1

公开(公告)日：2022-04-14

申请号：US17431219

申请日：2020-02-24

Applicant: Cytiva Sweden AB

Inventor： Matthew Townsend ,  Ian Scanlon

IPC: B01D15/18 ,  B01D15/12 ,  B01D15/34 ,  B01D15/38

Abstract: The present invention relates to a method for purifying a feed comprising at least one target product in a chromatography system having a plurality of purifying units, each having an inlet and an outlet, and a valve assembly having an outlet port and an inlet port. The inlet and the outlet of each purifying unit being connected to a respective port of the valve assembly. The method comprising loading (S10) the plurality of purifying units with feed provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: eluting (S12) the plurality of purifying units using an elution provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: and collecting (S14) the at least one target product from the outlet port of the valve.