公开(公告)号：US20230333098A1

公开(公告)日：2023-10-19

申请号：US18317411

申请日：2023-05-15

Applicant: Cytiva Sweden AB

Inventor： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC: G01N33/543 ,  C12M3/06 ,  B03C1/30 ,  C12M1/00 ,  C12M3/00 ,  B01D21/00 ,  C12N13/00 ,  B03C5/00 ,  B03C5/02 ,  C12M1/34 ,  B03C1/28 ,  B03C1/01

CPC classification number: G01N33/54326 ,  C12M27/16 ,  B03C1/30 ,  C12M45/07 ,  C12M23/48 ,  B01D21/0009 ,  C12M47/04 ,  C12N13/00 ,  C12M47/10 ,  B03C5/00 ,  C12M29/00 ,  B03C5/02 ,  C12M41/44 ,  G01N33/5436 ,  B03C1/288 ,  B03C1/01 ,  G01N2333/31 ,  B03C2201/26

Abstract: A separation system, and an elution arrangement to be provided in a separation system, for separating a biomolecule from a cell culture are provided.

公开(公告)号：US20220280886A1

公开(公告)日：2022-09-08

申请号：US17619204

申请日：2020-06-16

Applicant: Cytiva Sweden AB

Inventor： Gunnar Malmquist ,  Ola Lind ,  Matthew Townsend ,  Ian Scanlon ,  Zachary Michael Sexton

IPC: B01D15/18 ,  G01N30/86

Abstract: The present invention relates to a method for monitoring operational status in a column capture chromatography system configured for cyclical repetitive purification performed on a volume of sample feed comprising at least one product configured to be captured in the column during loading. The method comprises: performing (51) a purification cycle; measuring (52) at least one parameter during the purification cycle indicative of breakthrough of the at least one captured product after the column during loading of sample feed; when breakthrough is detected, reducing (56a) the amount of sample feed loaded during loading in the next purification cycle; and repeating the steps to perform another purification cycle. Each purification cycle comprising: loading an amount of sample feed onto the column, washing the column and eluting the at least one product.

公开(公告)号：US20220229052A1

公开(公告)日：2022-07-21

申请号：US17437118

申请日：2020-03-23

Applicant: Cytiva Sweden AB

Inventor： Ola Lind ,  Nils Norrman ,  Sara Häggblad Sahlberg ,  Ronnie Palmgren

IPC: G01N33/543 ,  G01N30/92

Abstract: The present disclosure is directed to a method for separating a biomolecule from a cell culture or from a biological solution, comprising the steps of: (a) providing magnetic particles comprising ligands capable of binding the biomolecule; (b) contacting a cell culture or a biological solution comprising the biomolecule with the magnetic particles to obtain magnetic particles comprising the bound biomolecule; (c) retaining the magnetic particles with a magnetic field in a magnetic separator; (d) optionally washing the magnetic particles with a washing liquid; (e1) providing a flow of an elution liquid through the magnetic separator to elute the bound biomolecule from the magnetic particles while retaining the magnetic particles with the magnetic field in the magnetic separator; (f1) forwarding the biomolecule eluted from the magnetic separator to a membrane chromatography device; (g1) separating the biomolecule from impurities and/or contaminants by membrane chromatography.