公开(公告)号：US20220280886A1

公开(公告)日：2022-09-08

申请号：US17619204

申请日：2020-06-16

Applicant: Cytiva Sweden AB

Inventor： Gunnar Malmquist ,  Ola Lind ,  Matthew Townsend ,  Ian Scanlon ,  Zachary Michael Sexton

IPC: B01D15/18 ,  G01N30/86

Abstract: The present invention relates to a method for monitoring operational status in a column capture chromatography system configured for cyclical repetitive purification performed on a volume of sample feed comprising at least one product configured to be captured in the column during loading. The method comprises: performing (51) a purification cycle; measuring (52) at least one parameter during the purification cycle indicative of breakthrough of the at least one captured product after the column during loading of sample feed; when breakthrough is detected, reducing (56a) the amount of sample feed loaded during loading in the next purification cycle; and repeating the steps to perform another purification cycle. Each purification cycle comprising: loading an amount of sample feed onto the column, washing the column and eluting the at least one product.

公开(公告)号：US20210269888A1

公开(公告)日：2021-09-02

申请号：US17253067

申请日：2019-07-01

Applicant: Cytiva Sweden AB

Inventor： Key Hyckenberg ,  Helena Skoglar ,  Gunnar Malmquist ,  Mikael Carlsten ,  Roger Lundqvist ,  Dan Andersson ,  Linda Mathiasson ,  Lars Mattsson ,  Sara Sendabo ,  Mikael Berg

IPC: C12Q3/00 ,  C12M1/36 ,  C12M1/00 ,  C12M1/34

Abstract: The present invention relates to a method for optimizing a bioprocess purification system comprising a bioreactor configured to provide a harvest comprising a target composition, and a purification process arranged downstream the bioreactor and being configured for purification of the harvest to produce a target product having a desired characteristics. The method comprising: a) detecting (32) at least one quality attribute indicative of characteristics of the target product in a downstream process, b) identifying (33) correlations between the at least one quality attribute measured in the downstream process and parameters to control a cell culture process in the bioreactor, and c) controlling (34) the cell culture process to meet the desired characteristics based on the identified correlations, whereby the target characteristics is within a pre-determined range.