公开(公告)号：US20210261900A1

公开(公告)日：2021-08-26

申请号：US17268486

申请日：2019-08-20

Applicant: Cytiva Sweden AB

Inventor： Anoop Bhargav ,  Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Pradeep Kumar ,  Victor Jose ,  Sahebagouda Alagur ,  Melissa Marie Semple ,  Mark David Roberts

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/34 ,  B01D65/02 ,  B01D61/20 ,  B01D61/10

Abstract: A method includes inducing a first quantity of a feed fluid (28) to flow along a first direction (72) from a bioreactor (12) to a tangential flow filter (16) to separate the first quantity of the feed fluid (28) into a permeate fluid (42) and a retentate fluid (48). Further, the control unit (66) is operated to control at least one feed flow control device (24, 26) to inhibit the flow of first quantity of the feed fluid (28). Furthermore, the control unit (66) is operated to control the at least one feed flow control device (24, 26) to direct at least one of further flow of a second quantity of the feed fluid (28) from the bioreactor (12), a portion of the permeate fluid (42), and a portion of a nutrient fluid, along a second direction (74) opposite to the first direction (72) via the tangential flow filter (16).