公开(公告)号：US20210395664A1

公开(公告)日：2021-12-23

申请号：US17277094

申请日：2019-09-20

Applicant: Cytiva Sweden AB

Inventor： Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Sahebagouda Alagur

IPC: C12M1/00 ,  B01D61/22 ,  B01D61/12 ,  C12M1/34

Abstract: A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

公开(公告)号：US20250092349A1

公开(公告)日：2025-03-20

申请号：US18962844

申请日：2024-11-27

Applicant: Cytiva Sweden AB

Inventor： Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Sahebagouda Alagur

IPC: C12M1/00 ,  B01D61/12 ,  B01D61/22 ,  C12M1/34

Abstract: A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path.

公开(公告)号：US12195715B2

公开(公告)日：2025-01-14

申请号：US17277094

申请日：2019-09-20

Applicant: Cytiva Sweden AB

Inventor： Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Sahebagouda Alagur

IPC: B01D61/12 ,  B01D61/22 ,  C12M1/00 ,  C12M1/34

Abstract: A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

公开(公告)号：US20230031710A1

公开(公告)日：2023-02-02

申请号：US17782591

申请日：2020-12-17

Applicant: CYTIVA SWEDEN AB

Inventor： Praveen Paul ,  Sahebagouda Alagur ,  Thomas Falkman ,  Prashanth Hosabettu Mohan

IPC: C12M1/00 ,  C12M1/34

Abstract: Systems (700) and methods (800) for method of continuous fluid flow in a bioreactor (700) is provided. The method (800) comprises providing (805) a bioreactor system (700) including a bioreactor volume (720), a filtration part (730), and a recirculation line (721) including a recirculation pump (722) is provided between the bioreactor volume (720) and the filtration part (730). The method (800) further comprises providing (810) a plurality of sensors (760) along the recirculation line (721) and monitoring the fluid flow parameters using the sensors (760). The method further comprises sending (820) a plurality of signals from the sensors (760) indicative of the fluid flow parameters to one or more controllers; and controlling (830) the fluid flow rate at the recirculation pump (722) by means of the or each controller.

公开(公告)号：US20220403318A1

公开(公告)日：2022-12-22

申请号：US17763860

申请日：2020-10-01

Applicant: Cytiva Sweden AB

Inventor： Shivakumar Selvaraj ,  Bineesh Kandoth ,  Sahebagouda Alagur ,  Nagaraj Raghavendra Rao ,  Thomas Falkman

IPC: C12M1/36 ,  C12M1/34 ,  C12M1/00

Abstract: The present subject matter discloses a bioreactor apparatus. The bioreactor apparatus comprises a bioreactor vessel configured to culture cells. The bioreactor apparatus furthermore comprises a sensor configured to measure Dissolved Oxygen (DO) in the bioreactor vessel. The DO measurements comprise a plurality of DO values recorded at, at least, a plurality of time instances during operation of the bioreactor apparatus. The bioreactor apparatus furthermore comprises a controller configured to obtain the DO measurements. The controller furthermore is to determine, in real-time or approximately real-time, an oxygen mass transfer co-efficient (kLa) associated with the operation of the bioreactor apparatus. Furthermore, the controller is configured to control, in real-time or approximately real-time, at least one cell culture parameter associated with the operation of the bioreactor apparatus based on the kLa.

公开(公告)号：US20210261900A1

公开(公告)日：2021-08-26

申请号：US17268486

申请日：2019-08-20

Applicant: Cytiva Sweden AB

Inventor： Anoop Bhargav ,  Praveen Paul ,  Prashanth Hosabettu Mohan ,  Ajay Gore ,  Pradeep Kumar ,  Victor Jose ,  Sahebagouda Alagur ,  Melissa Marie Semple ,  Mark David Roberts

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/34 ,  B01D65/02 ,  B01D61/20 ,  B01D61/10

Abstract: A method includes inducing a first quantity of a feed fluid (28) to flow along a first direction (72) from a bioreactor (12) to a tangential flow filter (16) to separate the first quantity of the feed fluid (28) into a permeate fluid (42) and a retentate fluid (48). Further, the control unit (66) is operated to control at least one feed flow control device (24, 26) to inhibit the flow of first quantity of the feed fluid (28). Furthermore, the control unit (66) is operated to control the at least one feed flow control device (24, 26) to direct at least one of further flow of a second quantity of the feed fluid (28) from the bioreactor (12), a portion of the permeate fluid (42), and a portion of a nutrient fluid, along a second direction (74) opposite to the first direction (72) via the tangential flow filter (16).