公开(公告)号：US20240279637A1

公开(公告)日：2024-08-22

申请号：US18693116

申请日：2022-09-30

Applicant: Cytiva Sweden AB

Inventor： Hans Blom ,  Markus Pitkanen ,  Linda Hagman ,  Annika Morrison

IPC: C12N15/10 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12N15/1003 ,  C12M23/14 ,  C12M41/12 ,  C12M41/26 ,  C12M47/06

Abstract: A method, a system and a use of a single use mixing bag for performing alkaline lysis, neutralisation and clarification of bacterial cells for extraction of plasmid DNA. The method comprises providing a suspension of bacterial cells and providing alkaline lysis solution into a single use mixing bag for performing an alkaline lysis of said bacterial cells, wherein said single use mixing bag comprises an integrated mixer. The method comprises furthermore mixing of the content in the single use mixing bag by the integrated mixer during the alkaline lysis and stopping the alkaline lysis by the addition of a neutralisation solution into the single use mixing bag and clarifying the content in the single use mixing bag after the alkaline lysis has been performed by the addition of a flocculate lifting agent into the single use mixing bag, whereby at least two phases are separated inside the single use mixing bag, whereby one of said phases is a neutralised and clarified alkaline solution comprising plasmid DNA. The neutralised and clarified alkaline solution is removed from the single use mixing bag and filtered.

公开(公告)号：US20220032301A1

公开(公告)日：2022-02-03

申请号：US17277046

申请日：2019-09-27

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Markus Pitkanen

IPC: B01L3/00

Abstract: The present disclosure relates to a biological fluid processing system and method. The system comprises a fluid processing device comprising at least one fluid path, a pump for providing a pressure in the at least one fluid path, a valve arranged along said fluid path and a first actuator arranged to control the valve to assume a desired opening state of said fluid path. The biological fluid processing system comprises further a processing interface comprising a pump drive for driving the pump of the fluid processing device, and a processing control element comprising a pump control system arranged to control at least the pump drive and a valve control system arranged to control the first actuator. The system is modular. The fluid processing device is comprised in a fluid processing device module having a predetermined fluid processing device configuration. The processing interfaces have a predetermined processing interface configuration. The processing control element is arranged to receive information relating to the predetermined processing interface configuration of the processing interface module and/or the predetermined fluid processing device configuration of the fluid processing device module and control the at least one pump drive and/or the valve based on the received information relating to the predetermined fluid processing device configuration and the predetermined processing interface configuration.