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公开(公告)号：US09600874B2
公开(公告)日：2017-03-21
申请号：US14275949
申请日：2014-05-13
发明人： Hiroki Fujimoto , Sanzo Moriwaki
CPC分类号： G06T7/0012 , G06K9/00134 , G06K9/03 , G06T2207/10056 , G06T2207/20021 , G06T2207/30024
摘要： A culture quality evaluation method comprises: a first step of culturing a pluripotent stem cell under a predetermined culture condition and creating a sample; a second step of imaging a proliferated cell colony in the sample and accordingly capturing an original image; a third step of dividing the original image into smaller images of a predetermined size and calculating standard deviations of pixel values of pixels of the smaller images; and a fourth step of judging whether the sample is an acceptable sample based upon a ratio of a number of the smaller images whose values of the standard deviations are within a predetermined range to a total number of the smaller images.
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公开(公告)号：US09204105B2
公开(公告)日：2015-12-01
申请号：US13793079
申请日：2013-03-11
CPC分类号： H04N7/18 , G01N21/253 , G01N21/255 , G01N2201/0631 , G01N2201/0634 , G01N2201/067
摘要： A preliminary image is obtained by irradiating illumination light, whose light quantity distribution is set at a standard state, to a specimen obtained by injecting fluid into a well from an illuminator capable of arbitrarily setting the light quantity distribution, for example, by a liquid crystal shutter, and a luminance distribution of the preliminary image is detected. As a result, luminance nonuniformity in the preliminary image is canceled by performing imaging with a light quantity distribution of illumination light to be incident on the well set such that an incident light amount is larger in a part with lower luminance.
摘要翻译： 通过将其光量分布设定为标准状态的照明光照射到能够从能够任意设定光量分布的照明装置将液体注入到井中而获得的试样，获得初步图像，例如通过液晶 快门，并且检测到初步图像的亮度分布。 结果，通过利用入射到套筒上的照明光的光量分布进行成像，使得入射光量在具有较低亮度的部分中较大，来抵消预备图像中的亮度不均匀性。
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公开(公告)号：US09176132B2
公开(公告)日：2015-11-03
申请号：US14169827
申请日：2014-01-31
发明人： Hiroki Fujimoto , Sanzo Moriwaki
IPC分类号： G01N21/00 , G01N33/569 , G01N21/59
CPC分类号： G01N33/569 , G01N21/5907 , G01N2021/5969
摘要： The following processes are performed to improve the accuracy of the process of estimating the volume of a cell clump from an image including the cell clump. First, the image including the cell clump is acquired, and the optical density of the cell clump in the image is measured. Cross-section information about the cell clump is acquired by observation using a confocal microscope or by physical cutting. Based on the cross-section information, the vertical height of the cell clump is determined. Thereafter, data representing a relationship between the aforementioned optical density and the height is acquired. This improves the accuracy of the process of converting the optical density into the height to thereby achieve the accurate estimation of the volume of the cell clump.
摘要翻译： 执行以下处理以提高从包括细胞团的图像估计细胞团块的体积的过程的准确性。 首先，获取包含细胞团的图像，测定图像中的细胞团的光密度。 通过使用共焦显微镜观察或通过物理切割获得关于细胞团的横截面信息。 基于横截面信息，确定细胞团块的垂直高度。 此后，获取表示上述光密度和高度之间的关系的数据。 这提高了将光密度转换成高度的过程的精度，从而实现细胞团的体积的准确估计。
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公开(公告)号：US09639736B2
公开(公告)日：2017-05-02
申请号：US14065484
申请日：2013-10-29
发明人： Hiroki Fujimoto , Jiro Tsumura
IPC分类号： G06K9/00
CPC分类号： G06K9/00147
摘要： An image processing apparatus displays an object adopted and an object not adopted by an adoption/non-adoption process in a distinguishable manner. A user designates an object whose adoption/non-adoption result is desired to be reversed among the objects displayed by the image processing apparatus. The image processing apparatus changes an allowable range stored in a storage part so that the adoption/non-adoption result of the designated object is reversed. That is, the user views the adoption/non-adoption result of the objects to change the allowable range of a parameter so that the adoption/non-adoption result becomes proper. This makes the allowable range of the parameter for use in the adoption/non-adoption process proper with ease.
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公开(公告)号：US09291551B2
公开(公告)日：2016-03-22
申请号：US14314603
申请日：2014-06-25
发明人： Sanzo Moriwaki , Hiroki Fujimoto
CPC分类号： G01N21/255 , G01N21/47 , G01N2201/064 , G01N2201/0668
摘要： An imaging apparatus for imaging a two-dimensional image of an imaging object comprises a holder which holds a sample container carrying a biological sample as the imaging object on a carrying surface, a light emitting part which emits light toward the carrying surface, an imager which includes a strip-like light receiving part, receives the light incident on the light receiving part and thereby images an image of a strip-like region of the carrying surface, a strip-like light shield which shields a part of light emitted from the illuminator toward the strip-like region, and a mover which integrally and relatively moves the light emitting part, the light receiving part and the light shield with respect to the sample container.
摘要翻译： 用于对成像对象的二维图像进行成像的成像装置包括：保持器，其将携带作为成像对象的生物样本的样本容器保持在承载表面上;发光部件，朝着承载表面发射光;成像器， 包括条状光接收部分，接收入射在光接收部分上的光，从而对承载表面的条状区域的图像进行成像;带状遮光罩，其屏蔽从照射器发出的光的一部分 朝向条状区域，以及使发光部，光接收部和遮光板相对于样本容器一体地移动的动子。
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公开(公告)号：US09292730B2
公开(公告)日：2016-03-22
申请号：US14150338
申请日：2014-01-08
发明人： Hiroki Fujimoto , Jiro Tsumura
IPC分类号： G06K9/00
CPC分类号： G06K9/00147
摘要： Switching between highlighting and unhighlighting of an object determined as adopted and switching between highlighting and unhighlighting of an object determined as not adopted are performed individually and independently by manipulation of an on/off selectable button. This allows switching to be freely performed between the following states in which: objects are highlighted regardless of whether the objects are adopted or not; only an object determined as adopted is highlighted; only an object determined as not adopted is highlighted; and objects are not highlighted regardless of whether the objects are adopted or not. A user visually judges whether the result of the adoption/non-adoption process is proper or not easily while switching between these displays.
摘要翻译： 通过开/关可选择按钮的操作，单独且独立地执行被确定为被采用的对象的高亮和不高亮之间的切换，以及被确定为未被采用的对象的高亮和非高亮度之间的切换。 这允许在以下状态之间自由执行切换，其中：无论是否采用对象，对象都被突出显示; 只有确定采用的对象被突出显示; 只有一个确定为不采用的对象被突出显示; 无论对象是否被采用，对象都不会突出显示。 在这些显示之间进行切换时，用户在视觉上判断采用/非采用处理的结果是否合适或不容易。
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