PROCESS FOR PRODUCING HIGH GLUCOSE COMPOSITIONS BY SIMULTANEOUS LIQUEFACTION AND SACCHARIFICATION OF STARCH SUBSTRATES
    1.
    发明申请
    PROCESS FOR PRODUCING HIGH GLUCOSE COMPOSITIONS BY SIMULTANEOUS LIQUEFACTION AND SACCHARIFICATION OF STARCH SUBSTRATES 审中-公开
    生产高葡萄糖组合物的方法同时进行液相色谱和淀粉底物的分离

    公开(公告)号:US20160108448A1

    公开(公告)日:2016-04-21

    申请号:US14649168

    申请日:2013-11-21

    Abstract: Fungal glucoamylases from Aspergillus fumigatus that are expressed in Trichoderma reesei host cells (AfGATR) are provided. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a simultaneous liquefaction and saccharification process in the presence of alpha amylase, such as Aspergillus kawachii alpha-amylase (AkAA). This greatly reduces the combined run time of liquefaction and saccharification reaction, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase.

    Abstract translation: 提供了在里氏木霉宿主细胞(AfGATR)中表达的来自烟曲霉的真菌葡糖淀粉酶。 包括AfGA1TR和AfGA2TR的AfGATR在高温和低pH下表现出高活性,因此在α淀粉酶如川曲霉α-淀粉酶(AkAA)的存在下,AfGATR可以有效地用于同时液化和糖化过程。 这大大降低了液化和糖化反应的组合运行时间,其中必须重新调整pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。

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