公开(公告)号：US09315831B2

公开(公告)日：2016-04-19

申请号：US13802420

申请日：2013-03-13

Applicant: Danisco US Inc. ,  The Goodyear Tire & Rubber Company

Inventor： Collette M. Blake ,  Gopal K. Chotani ,  Gang Duan ,  Douglas Ko ,  Floor Kooy ,  Sung Ho Lee ,  Michael J. Pepsin ,  Ying Qian ,  Matt Reboli ,  Vivek Sharma ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Pauline Johanna Maria Teunissen ,  Hongxian Xu

IPC: C12P5/02 ,  C12P5/00 ,  C12N9/88 ,  C12P7/42 ,  C12P7/46 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12P5/007 ,  C12N9/88 ,  C12P7/42 ,  C12P7/46 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12Y301/03 ,  Y02P20/52

Abstract: Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Abstract translation: 本文提供的组合物和方法与将研磨的或分级的颗粒中的淀粉直接转化成能够通过发酵生物体如异戊二烯工业生产一种或多种产物的碳源的可发酵糖原料有关， 类异戊二烯前体分子和/或类异戊二烯。 这样的转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。

公开(公告)号：US20190177757A1

公开(公告)日：2019-06-13

申请号：US16281989

申请日：2019-02-21

Applicant: DANISCO US INC

Inventor： Jing Ge ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

IPC: C12P19/14 ,  C12P19/02 ,  C12N9/34

CPC classification number: C12P19/14 ,  C12N9/2428 ,  C12P19/02 ,  C12Y302/01003 ,  Y02E50/17

Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

公开(公告)号：US20160108448A1

公开(公告)日：2016-04-21

申请号：US14649168

申请日：2013-11-21

Applicant: DANISCO US INC.

Inventor： Jing Ge ,  Ling Hua ,  Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Zhongmei Tang ,  Donald E. Ward

IPC: C12P19/16 ,  C12P19/02

CPC classification number: C12P19/16 ,  C12C5/004 ,  C12C12/00 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  C13K1/06

Abstract: Fungal glucoamylases from Aspergillus fumigatus that are expressed in Trichoderma reesei host cells (AfGATR) are provided. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a simultaneous liquefaction and saccharification process in the presence of alpha amylase, such as Aspergillus kawachii alpha-amylase (AkAA). This greatly reduces the combined run time of liquefaction and saccharification reaction, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase.

Abstract translation: 提供了在里氏木霉宿主细胞（AfGATR）中表达的来自烟曲霉的真菌葡糖淀粉酶。 包括AfGA1TR和AfGA2TR的AfGATR在高温和低pH下表现出高活性，因此在α淀粉酶如川曲霉α-淀粉酶（AkAA）的存在下，AfGATR可以有效地用于同时液化和糖化过程。 这大大降低了液化和糖化反应的组合运行时间，其中必须重新调整pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。

公开(公告)号：US20150037844A1

公开(公告)日：2015-02-05

申请号：US14225987

申请日：2012-09-07

Applicant: Danisco US Inc.

Inventor： Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12P19/20 ,  C12P19/02

CPC classification number: C12P19/20 ,  C12P19/02 ,  C12P19/14 ,  C12P19/16

Abstract: The present teachings provide methods of processing granular starch in slurries containing high dry solids content. The slurries are initially incubated with enzymes at or below the gelatinization temperature. The use of pullulanase and glucoamylase at specified doses allows for improved glucose yields at lower energy cost.

Abstract translation: 本教导提供了在含有高干固体含量的浆料中处理颗粒淀粉的方法。 浆料最初与等于或低于凝胶化温度的酶一起温育。 在特定剂量下使用支链淀粉酶和葡糖淀粉酶允许以更低的能量成本提高葡萄糖产量。

公开(公告)号：US10767207B2

公开(公告)日：2020-09-08

申请号：US16281989

申请日：2019-02-21

Applicant: DANISCO US INC

Inventor： Jing Ge ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

IPC: C12P19/14 ,  C12P19/02 ,  C12N9/30 ,  C12C5/00 ,  C11D3/386 ,  A21D8/04 ,  C12N9/34

Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

公开(公告)号：US20150152458A1

公开(公告)日：2015-06-04

申请号：US14479653

申请日：2013-03-26

Applicant: Danisco US Inc.

Inventor： Bart Christian Koops ,  Floor K. Kooy ,  Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Arjen Hendrik van Tuijl

IPC: C12P19/20

CPC classification number: C12P19/20 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01003 ,  C13K1/06

Abstract: The present teachings provide a method for making a high glucose syrup at a low temperature. In some embodiments, the syrup contains reduced reversion reaction products. The method comprises contacting a starch substrate at a temperature below the starch gelatinization temperature with an enzyme blend comprising a high dose of alpha-amylase and a low dose of glucoamylase. In some embodiments, a blend of two glucoamylases is employed. In some embodiments, a debranching enzyme such as a pullulanase is employed. In some embodiments, the enzymes are staged by adding at different times, or at different temperatures. The present teachings provide for high glucose syrups with fewer reversion products, and allow for higher starch solubilization.

Abstract translation: 本教导提供了一种在低温下制备高糖浆的方法。 在一些实施方案中，糖浆含有降低的逆转反应产物。 该方法包括在低于淀粉糊化温度的温度下将淀粉底物与包含高剂量的α-淀粉酶和低剂量葡糖淀粉酶的酶混合物接触。 在一些实施方案中，使用两种葡糖淀粉酶的共混物。 在一些实施方案中，使用脱支酶，例如支链淀粉酶。 在一些实施方案中，通过在不同时间或在不同的温度下加入来分级酶。 本教导提供具有较少逆转产物的高葡萄糖浆，并允许更高的淀粉溶解。

公开(公告)号：US20150152457A1

公开(公告)日：2015-06-04

申请号：US14479683

申请日：2013-03-13

Applicant: Danisco US Inc.

Inventor： Collette Michelle Blake ,  Gang Duan ,  Douglas Ko ,  Floor K. Kooy ,  Sung Ho Lee ,  Michael Jay Pepsin ,  Ying Qian ,  Matthew T. Reboli ,  Vivek Sharma ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Paula Johanna Maria Teunissen ,  Hongxian Xu

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P19/02 ,  C12P19/12 ,  C12P19/20 ,  C12Y301/03 ,  C12Y302/01001 ,  C12Y302/01003

Abstract: Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Abstract translation: 本文提供的是与将研磨或分馏的谷粒中的淀粉直接转化成可发酵糖原料相关的组合物和方法，其可以作为发酵生物体工业生产一种或多种产品的碳源。 这样的转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。

公开(公告)号：US20150111259A1

公开(公告)日：2015-04-23

申请号：US14387779

申请日：2013-03-12

Applicant: Danisco US Inc.

Inventor： T. Kleinhout ,  Bart Christiaan Koops ,  Floor K. Kooy ,  Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12P19/12 ,  C12P19/14

CPC classification number: C12P19/12 ,  A23L29/35 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01002 ,  C12Y302/01068 ,  C12Y302/01133 ,  C13K7/00

Abstract: The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same.

Abstract translation: 本教导提供将颗粒淀粉直接转化成包含高，非常高和/或超高麦芽糖含量的可溶性糖组合物。 该方法包括使颗粒状淀粉的含水浆液与包含α-淀粉酶和麦芽糖酶的合适比例的酶组合物接触，以产生酶促转化为含有较高麦芽糖的糖组合物的可溶性淀粉底物。 该过程可以使用多于一个温度来实现。

公开(公告)号：US20130288309A1

公开(公告)日：2013-10-31

申请号：US13857004

申请日：2013-04-04

Applicant: Danisco US Inc.

Inventor： Gang DUAN ,  Sung Ho Lee ,  Ying Qian ,  Rafael F. Sala ,  Jayarama Shetty

IPC: C12P19/14 ,  C12P19/16 ,  C12P7/14

CPC classification number: C12P19/14 ,  C12N9/2417 ,  C12P7/10 ,  C12P7/14 ,  C12P19/16 ,  D06L1/14 ,  D06M16/003 ,  Y02E50/16

Abstract: Variants of a Pseudomonas saccharophila G4-forming amylase (PS4) advantageously can catalyze high temperature saccharification to produce maltotetraose syrup from a starch liquefact or granular starch, e.g., derived from cornstarch. The PS4 variants are useful in a process of saccharification of starch that advantageously produces significant amounts of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.

Abstract translation: 酿酒酵母G4形成淀粉酶（PS4）的变体有利地可以催化高温糖化以从淀粉液化或颗粒淀粉（例如衍生自玉米淀粉）产生麦芽四糖糖浆。 PS4变体可用于淀粉糖化过程，其有利地产生显着量的麦芽四糖，其可以在生产麦芽四糖糖浆的方法中在下游使用。 在一个实施方案中，提供了基于总糖含量可产生约40重量％至约60重量％麦芽四糖的耐热性PS4变体。

公开(公告)号：US20210047664A1

公开(公告)日：2021-02-18

申请号：US17002091

申请日：2020-08-25

Applicant: DANISCO US INC

Inventor： JING GE ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K. Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02

Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.