公开(公告)号：US09115378B2

公开(公告)日：2015-08-25

申请号：US13654105

申请日：2012-10-17

Applicant: DSM IP Assets B.V.

Inventor： Marie-Gabrielle Beuzelin-Ollivier ,  Bastien Chevreux ,  Manuela Dalluege ,  Marina Van Gelder ,  Markus G. Goese ,  Corina Hauk ,  Bertus P. Koekman ,  Connie Lee ,  Anne F. Mayer ,  Anja Meury ,  Nigel J. Mouncey ,  Dick Schipper ,  Masako Shinjoh ,  Christine Toepfer ,  Adrianus W. H. Vollebregt

IPC: C12P17/04 ,  C12N9/02

CPC classification number: C12P17/04 ,  C12N9/0008

Abstract: The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

Abstract translation: 本发明涉及能够直接生产L-抗坏血酸（以下也称为维生素C）的新鉴定的微生物。 本发明还涉及包含参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽 以及它们的功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：US10793837B2

公开(公告)日：2020-10-06

申请号：US15579751

申请日：2016-06-06

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jun Wang ,  Bastien Chevreux ,  Ross E. Zirkle ,  Anne-Cecile Bayne ,  James Casey Lippmeier

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/00 ,  A23K20/158 ,  A23L33/115 ,  C12P7/64

Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US10081797B2

公开(公告)日：2018-09-25

申请号：US14650297

申请日：2013-08-27

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Farrell ,  Maria Mayorga ,  Bastien Chevreux

IPC: C12P23/00 ,  C12N15/52 ,  C12N9/02

CPC classification number: C12N9/0073 ,  C12P23/00 ,  C12Y114/13129

Abstract: The present invention relates to novel hydroxylases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith, processes for the microbiological hydroxylation of isoprenoids, for example processes for converting beta-carotene into beta-cryptoxanthin and zeaxanthin, or canthaxanthin into astaxanthin.