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公开(公告)号:US20190338214A1
公开(公告)日:2019-11-07
申请号:US16515074
申请日:2019-07-18
Applicant: DSM IP Assets B.V.
Inventor: Stephen Robert CHERINKO , Robert Cody KERTIS , Kirk E. APT , Paul Warren BEHRENS , Jon Milton HANSEN , Joseph W. PFEIFER, III , Tracy Lynn STAHL , Ross E. Zirkle , Nasrin TABAYEHNEJAD , Krishna RAMAN , Neil Francis LEININGER
Abstract: The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.
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公开(公告)号:US10793837B2
公开(公告)日:2020-10-06
申请号:US15579751
申请日:2016-06-06
Applicant: DSM IP Assets B.V.
Inventor: James G. Metz , Jun Wang , Bastien Chevreux , Ross E. Zirkle , Anne-Cecile Bayne , James Casey Lippmeier
IPC: C12N15/82 , C12N9/10 , C12N9/00 , A23K20/158 , A23L33/115 , C12P7/64
Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.
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公开(公告)号:US11987819B2
公开(公告)日:2024-05-21
申请号:US16300212
申请日:2017-05-11
Applicant: DSM IP Assets B.V.
Inventor: Anne-Cecile V. Bayne , Ross E. Zirkle
IPC: A01H5/00 , C12N9/10 , C12N15/00 , C12N15/82 , C12P7/6432 , C12P7/6472
CPC classification number: C12N9/1029 , A01H5/00 , C12N15/00 , C12N15/8247 , C12P7/6432 , C12P7/6472 , C12Y203/01085
Abstract: The subject disclosure features, in one aspect, a method for producing lipids enriched for EPA, comprising modifying a microalga to increase expression of PFA1, and/or PFA3, and culturing the modified microalga under conditions which allow the expression of PFA1, and/or PFA3, wherein lipids enriched for EPA are produced. Also featured is a recombinant microalga in which PFA1, and/or PFA3, is overexpressed. Such recombinant microalgae have been demonstrated herein to produce very favorable fatty acid lipid profiles (e.g., increased levels of EPA, increased ratio of EPA:DHA, decreased levels of DPA n-6, etc.).
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