公开(公告)号：US20190338214A1

公开(公告)日：2019-11-07

申请号：US16515074

申请日：2019-07-18

Applicant: DSM IP Assets B.V.

Inventor： Stephen Robert CHERINKO ,  Robert Cody KERTIS ,  Kirk E. APT ,  Paul Warren BEHRENS ,  Jon Milton HANSEN ,  Joseph W. PFEIFER, III ,  Tracy Lynn STAHL ,  Ross E. Zirkle ,  Nasrin TABAYEHNEJAD ,  Krishna RAMAN ,  Neil Francis LEININGER

IPC: C11B1/10 ,  C11B3/16 ,  C11B3/02

Abstract: The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

公开(公告)号：US10793837B2

公开(公告)日：2020-10-06

申请号：US15579751

申请日：2016-06-06

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jun Wang ,  Bastien Chevreux ,  Ross E. Zirkle ,  Anne-Cecile Bayne ,  James Casey Lippmeier

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/00 ,  A23K20/158 ,  A23L33/115 ,  C12P7/64

Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US11987819B2

公开(公告)日：2024-05-21

申请号：US16300212

申请日：2017-05-11

Applicant: DSM IP Assets B.V.

Inventor： Anne-Cecile V. Bayne ,  Ross E. Zirkle

IPC: A01H5/00 ,  C12N9/10 ,  C12N15/00 ,  C12N15/82 ,  C12P7/6432 ,  C12P7/6472

CPC classification number: C12N9/1029 ,  A01H5/00 ,  C12N15/00 ,  C12N15/8247 ,  C12P7/6432 ,  C12P7/6472 ,  C12Y203/01085

Abstract: The subject disclosure features, in one aspect, a method for producing lipids enriched for EPA, comprising modifying a microalga to increase expression of PFA1, and/or PFA3, and culturing the modified microalga under conditions which allow the expression of PFA1, and/or PFA3, wherein lipids enriched for EPA are produced. Also featured is a recombinant microalga in which PFA1, and/or PFA3, is overexpressed. Such recombinant microalgae have been demonstrated herein to produce very favorable fatty acid lipid profiles (e.g., increased levels of EPA, increased ratio of EPA:DHA, decreased levels of DPA n-6, etc.).