公开(公告)号：US09518102B2

公开(公告)日：2016-12-13

申请号：US14548054

申请日：2014-11-19

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C12N15/62 ,  C07K14/705 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90 ,  C12N15/79

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US09012616B2

公开(公告)日：2015-04-21

申请号：US14108794

申请日：2013-12-17

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C07H21/04 ,  C12N1/13 ,  C12P21/02 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US10829794B2

公开(公告)日：2020-11-10

申请号：US16249253

申请日：2019-01-16

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C07H21/04 ,  C12P21/00 ,  C07K14/37 ,  C12N15/80 ,  C12R1/90 ,  C07K14/705 ,  C12N15/79 ,  C12N9/26

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

公开(公告)号：US10793837B2

公开(公告)日：2020-10-06

申请号：US15579751

申请日：2016-06-06

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jun Wang ,  Bastien Chevreux ,  Ross E. Zirkle ,  Anne-Cecile Bayne ,  James Casey Lippmeier

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/00 ,  A23K20/158 ,  A23L33/115 ,  C12P7/64

Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.