公开(公告)号：US20170218468A1

公开(公告)日：2017-08-03

申请号：US15119708

申请日：2015-02-20

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs KOUWEN ,  Pim VAN HEE ,  Douwe VAN SINDEREN ,  Brian MCDONNELL ,  Jennifer MAHONY

IPC: C12R1/46 ,  A23C19/032 ,  G06F19/22 ,  A23C9/123 ,  C12N1/20 ,  C12N1/04

CPC classification number: C12R1/46 ,  A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/04 ,  C12N1/20 ,  C12N15/01 ,  C12Q1/689 ,  G06F19/22

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.

公开(公告)号：US20170196233A1

公开(公告)日：2017-07-13

申请号：US15327661

申请日：2015-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs KOUWEN ,  Laurens Leendert HANEMAAIJER ,  Douwe VAN SINDEREN ,  Brian MCDONNELL ,  Jennifer MAHONY

IPC: A23C9/123 ,  A23C19/032 ,  C12N15/90 ,  C12N15/74 ,  C12N15/113

CPC classification number: A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/20 ,  C12N1/36 ,  C12N15/113 ,  C12N15/746 ,  C12N15/902 ,  C12N2310/11

Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.