公开(公告)号：US20170218468A1

公开(公告)日：2017-08-03

申请号：US15119708

申请日：2015-02-20

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs KOUWEN ,  Pim VAN HEE ,  Douwe VAN SINDEREN ,  Brian MCDONNELL ,  Jennifer MAHONY

IPC: C12R1/46 ,  A23C19/032 ,  G06F19/22 ,  A23C9/123 ,  C12N1/20 ,  C12N1/04

CPC classification number: C12R1/46 ,  A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/04 ,  C12N1/20 ,  C12N15/01 ,  C12Q1/689 ,  G06F19/22

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.

公开(公告)号：US20160017408A1

公开(公告)日：2016-01-21

申请号：US14773065

申请日：2014-03-13

Applicant: DSM IP ASSETS B.V.

Inventor： Douwe VAN SINDEREN ,  Finn VOGENSEN ,  Jennifer MAHONY ,  Witold KOT ,  Stuart AINSWORTH

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: A kit useful for determining the phage susceptibility of one or more strains of Lactococcus lactis is described. The disclosure also describes methods for formulation of mixed defined starter cultures using strains from different phage sensitivity groups.

Abstract translation: 描述了可用于确定一种或多种乳酸乳球菌菌株的噬菌体易感性的试剂盒。 本公开还描述了使用来自不同噬菌体敏感性组的菌株制备混合定义的起始培养物的方法。

公开(公告)号：US20170196233A1

公开(公告)日：2017-07-13

申请号：US15327661

申请日：2015-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs KOUWEN ,  Laurens Leendert HANEMAAIJER ,  Douwe VAN SINDEREN ,  Brian MCDONNELL ,  Jennifer MAHONY

IPC: A23C9/123 ,  A23C19/032 ,  C12N15/90 ,  C12N15/74 ,  C12N15/113

CPC classification number: A23C9/1238 ,  A23C19/0323 ,  A23Y2240/75 ,  C12N1/20 ,  C12N1/36 ,  C12N15/113 ,  C12N15/746 ,  C12N15/902 ,  C12N2310/11

Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.