公开(公告)号：US20150064765A1

公开(公告)日：2015-03-05

申请号：US14394771

申请日：2013-04-23

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries Roubos ,  Jan Metske Van Der Laan ,  Bastiaan Van Den Berg ,  Dick De Ridder

IPC: C12N9/42 ,  C12N9/30

CPC classification number: C12N9/2437 ,  C12N9/242 ,  C12N15/67 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y302/01004

Abstract: A method for the production of a polypeptide of interest in a host cell, which method comprises: a. providing a host cell which harbours a nucleic acid encoding a polypeptide of interest, wherein the polypeptide of interest is modified so that it comprises fewer methionine and/or lysine residues than a reference polypeptide, excluding any initial methionine amino acid located at the N-terminal end of the polypeptide sequence; b. cultivating the host cell under conditions suitable for production of the polypeptide; and, optionally, c. recovering the compound of interest. The invention also relates to a modified polypeptide which comprises fewer methionine and/or lysine residues than a reference polypeptide, excluding any initial methionine amino acid located at the N-terminal end of the polypeptide sequence.

Abstract translation: 一种在宿主细胞中产生感兴趣的多肽的方法，该方法包括：a。 提供宿主细胞，其含有编码感兴趣的多肽的核酸，其中所述目的多肽被修饰，使得其包含比参考多肽更少的甲硫氨酸和/或赖氨酸残基，不包括位于N端的任何初始甲硫氨酸氨基酸 多肽序列的末端; b。 在适于生产多肽的条件下培养宿主细胞; 和可选地，c。 回收目的化合物。 本发明还涉及一种修饰的多肽，其包含比参考多肽更少的甲硫氨酸和/或赖氨酸残基，不包括位于多肽序列的N-末端的任何初始甲硫氨酸氨基酸。