公开(公告)号：US20150064765A1

公开(公告)日：2015-03-05

申请号：US14394771

申请日：2013-04-23

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries Roubos ,  Jan Metske Van Der Laan ,  Bastiaan Van Den Berg ,  Dick De Ridder

IPC: C12N9/42 ,  C12N9/30

CPC classification number: C12N9/2437 ,  C12N9/242 ,  C12N15/67 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y302/01004

Abstract: A method for the production of a polypeptide of interest in a host cell, which method comprises: a. providing a host cell which harbours a nucleic acid encoding a polypeptide of interest, wherein the polypeptide of interest is modified so that it comprises fewer methionine and/or lysine residues than a reference polypeptide, excluding any initial methionine amino acid located at the N-terminal end of the polypeptide sequence; b. cultivating the host cell under conditions suitable for production of the polypeptide; and, optionally, c. recovering the compound of interest. The invention also relates to a modified polypeptide which comprises fewer methionine and/or lysine residues than a reference polypeptide, excluding any initial methionine amino acid located at the N-terminal end of the polypeptide sequence.

Abstract translation: 一种在宿主细胞中产生感兴趣的多肽的方法，该方法包括：a。 提供宿主细胞，其含有编码感兴趣的多肽的核酸，其中所述目的多肽被修饰，使得其包含比参考多肽更少的甲硫氨酸和/或赖氨酸残基，不包括位于N端的任何初始甲硫氨酸氨基酸 多肽序列的末端; b。 在适于生产多肽的条件下培养宿主细胞; 和可选地，c。 回收目的化合物。 本发明还涉及一种修饰的多肽，其包含比参考多肽更少的甲硫氨酸和/或赖氨酸残基，不包括位于多肽序列的N-末端的任何初始甲硫氨酸氨基酸。

公开(公告)号：US10316306B2

公开(公告)日：2019-06-11

申请号：US15501149

申请日：2015-08-06

Applicant: DSM IP ASSETS B.V.

Inventor： Hanna Elisabet Abbas ,  Jan Metske Van Der Laan ,  Johannes Gustaaf Ernst Van Leeuwen

IPC: C12N9/12 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/30 ,  A21D2/26

Abstract: The present invention relates to an alpha amylase enzyme and to its use in the food, feed and non-food industry.

公开(公告)号：US10202594B2

公开(公告)日：2019-02-12

申请号：US15314892

申请日：2015-06-03

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus

IPC: C12N9/50 ,  C12N9/62

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one combination of amino acid substitutions selected from the group consisting of a combination selected from the group consisting of (K238E, I204V, V460A), (F279S, A242V, N507I), (T145A, K424M), (T359A, F379S), (M170I, A421T), (N441S, G484S), (L470H, Q288R), (L470H, E387G), (T281S, L373I), (P304A, P469A), and (P466T, P469Q), a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.

公开(公告)号：US09127266B2

公开(公告)日：2015-09-08

申请号：US14075711

申请日：2013-11-08

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Mark Cristiaan Stor ,  Ilse De Lange ,  Lisette Mohrmann

IPC: C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  A61K38/46 ,  C12N9/82 ,  A23L1/015 ,  A23L1/03

CPC classification number: C12N9/82 ,  A23L5/25 ,  A23L29/06 ,  C12Y305/01001

Abstract: The present invention relates to newly identified asparaginase polypeptide variants of SEQ ID NO: 3 and to polynucleotide sequences that encode such novel asparaginase variants. Furthermore the invention relates to the use of these novel asparaginase variants in industrial processes.

Abstract translation: 本发明涉及新鉴定的SEQ ID NO：3的天冬酰胺酶多肽变体和编码这种新型天冬酰胺酶变体的多核苷酸序列。 此外，本发明涉及这些新型天冬酰胺酶变体在工业过程中的用途。

公开(公告)号：US10450554B2

公开(公告)日：2019-10-22

申请号：US15312171

申请日：2015-05-19

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus ,  Johanna Henrica Gerdina Maria Mutsaers

IPC: A61K38/48 ,  C12N1/20 ,  C12N9/62 ,  A23L2/84 ,  A23J3/34 ,  C12N9/50 ,  A23L2/66 ,  A23L7/10 ,  A23L33/18 ,  A23L33/195

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.

公开(公告)号：US09307776B2

公开(公告)日：2016-04-12

申请号：US14168593

申请日：2014-01-30

Applicant: DSM IP ASSETS B.V.

Inventor： Lucie Parenicova ,  Ronald Busink ,  Jan Metske Van Der Laan

IPC: C12N9/20 ,  A23C19/06 ,  A23C19/032 ,  A23C19/072 ,  A23C19/082

CPC classification number: A23C19/063 ,  A23C19/0328 ,  A23C19/072 ,  A23C19/082 ,  C12N9/20

Abstract: The present invention relates to novel lipase polynucleotide sequences, their corresponding proteins as well as ways of manufacturing said sequences and said proteins and use of the proteins in the preparation of food compositions. The invention further relates to methods for releasing proteins from the exterior of a host cell as well as to a method for killing micro-organisms.

Abstract translation: 本发明涉及新的脂肪酶多核苷酸序列，其相应的蛋白质以及制备所述序列和所述蛋白质的方法以及蛋白质在制备食物组合物中的用途。 本发明还涉及从宿主细胞外部释放蛋白质的方法以及杀死微生物的方法。

公开(公告)号：US10294467B2

公开(公告)日：2019-05-21

申请号：US15314825

申请日：2015-06-03

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus

IPC: C12N9/62 ,  C12C11/00

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.