公开(公告)号：US20250128210A1

公开(公告)日：2025-04-24

申请号：US18569986

申请日：2022-06-14

Applicant: DSM IP ASSETS B.V.

Inventor： Nikolay KHANZHIN ,  Pierre CHASSAGNE

IPC: B01D61/58 ,  B01D61/02 ,  B01D61/04

Abstract: The invention relates to a method for recovery and purification of human milk oligosaccharides (HMOs) from a fermentation broth, comprising the steps of separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream, purifying the separated HMO-containing stream, concentrating the purified HMO-containing stream, and drying the purified HMO-containing stream to obtain solidified HMO. Moreover, the invention also concerns a human milk oligosaccharide obtained by the inventive method, as well as its use in food, feed, and medical application.

公开(公告)号：US20240349770A1

公开(公告)日：2024-10-24

申请号：US18557902

申请日：2022-04-28

Applicant: DSM IP Assets B.V.

Inventor： Nikolay KHANZHIN ,  Pierre CHASSAGNE

IPC: A23L33/00 ,  A23L33/12 ,  A23L33/125 ,  A23L33/155

CPC classification number: A23L33/40 ,  A23L33/12 ,  A23L33/125 ,  A23L33/155

Abstract: Infant formulae with a very low level of arsenic are obtained by selecting the components of the infant formula very carefully. For manufacturing infant formulae that comprise less than 95 parts per billion (ppb) of arsenic, it is preferred to use human milk oligosaccharides (HMOs) that have been isolated from a fermentation brth without using activated carbon in the last purification step. The preferred HMO is 2′-O-fucosyllactose.

公开(公告)号：US20240286081A1

公开(公告)日：2024-08-29

申请号：US18570003

申请日：2022-06-14

Applicant: DSM IP ASSETS B.V.

Inventor： Nikolay KHANZHIN ,  Pierre CHASSAGNE

IPC: B01D61/02 ,  B01D61/14 ,  B01D61/58

CPC classification number: B01D61/029 ,  B01D61/025 ,  B01D61/0271 ,  B01D61/145 ,  B01D61/58 ,  B01D2311/18 ,  B01D2311/2623 ,  B01D2311/2673 ,  B01D2311/2699 ,  B01D2315/16

Abstract: The invention relates to a method for recovery and purification of a neutral or sialylated human milk oligosaccharide (HMO) from a fermentation broth, comprising the steps of separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream, purifying the HMO-containing stream by ultrafiltration using an ultrafiltration membrane having a MWCO of 500 Da to 5 kDa, purifying the HMO-containing stream by nanofiltration, concentrating the purified HMO-containing stream, and drying the purified HMO-containing stream to obtain a solidified neutral or sialylated HMO. Moreover, the invention also concerns a neutral or sialylated human milk oligosaccharide obtained by the inventive method, as well as its use in food, feed, and medical application.

公开(公告)号：US20240287116A1

公开(公告)日：2024-08-29

申请号：US18570021

申请日：2022-06-14

Applicant: DSM IP ASSETS B.V.

Inventor： Nikolay KHANZHIN ,  Pierre CHASSAGNE

IPC: C07H3/06 ,  B01J47/014 ,  C07H1/08

CPC classification number: C07H3/06 ,  B01J47/014 ,  C07H1/08

Abstract: The invention relates to a method for recovery and purification of a neutral or sialylated human milk oligosaccharide (HMO) from a fermentation broth, comprising the steps of separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream, purifying the HMO-containing stream by nanofiltration, purifying the HMO-containing stream with an acidic cation exchange resin, concentrating the purified HMO-containing stream, and drying the purified HMO-containing stream to obtain a solidified neutral or sialylated HMO. Moreover, the invention also concerns a neutral or sialylated human milk oligosaccharide obtained by the inventive method, as well as its use in food, feed, and medical application.

公开(公告)号：US20230074506A1

公开(公告)日：2023-03-09

申请号：US17759590

申请日：2021-01-29

Applicant: DSM IP ASSETS B.V.

Inventor： Arthur Maurits Christiaan JANSE ,  Pierre CHASSAGNE

IPC: C12P19/04 ,  C07H3/06 ,  C07H1/08

Abstract: A process for recovery and purification of HMOs comprising: (a) providing an HMO-containing fermentation broth comprising biomass; (b) separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream; (c) purifying the separated HMO-containing stream; (d) concentrating the separated HMO-containing stream; and (e) drying the product of steps (a)-(d) by an indirect drying method thereby forming a purified HMO, wherein steps (c)-(d) can be performed in any order.