公开(公告)号：US20190010419A1

公开(公告)日：2019-01-10

申请号：US16130575

申请日：2018-09-13

Applicant: DSM IP Assets B.V.

Inventor： Kirk APT ,  William BARCLAY ,  Micah BLAZER ,  Jacob BORDEN ,  Adam Burja ,  Daniel DONG ,  Armando DURAZO ,  Jean-Charles DUMENIL ,  Arthur EDGE ,  Jon HANSEN ,  Alexandra HOFLER ,  David JEFFERS ,  Chris LYON ,  Vidya PAI ,  Joseph William PFEIFER, III ,  Martin J. SELLERS ,  Ginger SHANK ,  Justin STEGE

IPC: C11B1/02 ,  C10L1/02 ,  C12N9/26 ,  C12N1/06 ,  C12N9/24 ,  C11B1/12 ,  C11B1/10 ,  C12P7/64

Abstract: Methods and systems used to extract lipids suitable in production of biofuels from a fermentation broth may include using heat to pre-treat the fermentation broth in order to more easily extract a product from oleaginous microorganisms in the broth. Additionally or alternatively, a combination of enzymes including amylase, 1-4 mannosidase, and 1-3 mannosidase may be used to break down cell walls of the oleaginous microorganisms. Residual broth water may be recycled and used as imbibition water for washing a process feedstock to extract sugar.

公开(公告)号：US20190390135A1

公开(公告)日：2019-12-26

申请号：US16563260

申请日：2019-09-06

Applicant: DSM IP ASSETS B.V.

Inventor： Neil Francis LEININGER ,  Ginger SHANK ,  Xiao DONG ,  Joseph William PFEIFER, III ,  Vidya PAI

IPC: C11B1/02 ,  C11B3/00

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.