公开(公告)号：US20210017467A1

公开(公告)日：2021-01-21

申请号：US17042791

申请日：2019-03-29

Applicant: DSM IP Assets B.V. ,  Evonik Operations GmbH

Inventor： Negash ADUGNA ,  Martin HEINING ,  Michael Benjamin JOHNSON ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS, Sr. ,  Ginger Marie SHANK ,  Nasrin TABAYEHNEJAD ,  Vinod TARWADE

IPC: C11B1/02 ,  C11B1/10

Abstract: Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

公开(公告)号：US20190323043A1

公开(公告)日：2019-10-24

申请号：US16473805

申请日：2017-12-20

Applicant: EVONIK DEGUSSA GMBH ,  DSM IP ASSETS B.V.

Inventor： Michael DIEHL ,  Xiao Daniel DONG ,  Annika HARTMANN ,  Martin HEINING ,  Michael Benjamin JOHNSON ,  Jochen LEBERT ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS, Sr. ,  Mark Edward NEJAKO, II ,  Holger PFEIFER ,  Christian RABE ,  Shannon Elizabeth Ethier RESOP ,  Ginger Marie SHANK ,  Vinod TARWADE ,  David Allen TINSLEY

IPC: C12P7/64 ,  C11B1/10

Abstract: The current invention relates to a method of isolated polyunsaturated fatty acids containing lipids from a lipids containing biomass.

公开(公告)号：US20240409841A1

公开(公告)日：2024-12-12

申请号：US18809575

申请日：2024-08-20

Applicant: DSM IP Assets B.V.

Inventor： Neil Francis LEININGER ,  Ginger Marie SHANK ,  Xiao Daniel DONG ,  Joseph William PFEIFER, III ,  Vidya PAI

IPC: C11B1/02 ,  C07C51/42 ,  C07C51/487 ,  C11B3/00

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

公开(公告)号：US20200231898A1

公开(公告)日：2020-07-23

申请号：US16317305

申请日：2017-07-12

Applicant: Evonik Degussa GmbH ,  DSM IP Assets B.V.

Inventor： Manfred Barz ,  Marc BEISER ,  Georg BORCHERS ,  Stephen Robert CHERINKO ,  Mathias DERNEDDE ,  Michael DIEHL ,  Xiao Daniel DONG ,  Jügen HABERLAND ,  Michael Benjamin JOHNSON ,  Robert Cody KERTIS ,  Jochen LEBERT ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS ,  Holger PFEIFER ,  Christian RABE ,  Shannon Elizabeth Ethier RESOP ,  Ginger Marie SHANK ,  Vinod TARWADE ,  David Allen TINSLEY ,  Daniel VERKOEIJEN

IPC: C11B1/12 ,  C11B1/02 ,  A23K10/12 ,  A23K20/158

Abstract: The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

公开(公告)号：US20230242836A1

公开(公告)日：2023-08-03

申请号：US18010795

申请日：2021-06-10

Applicant: EVONIK OPERATIONS GMBH ,  DSM IP ASSETS B.V.

Inventor： Michael DIEHL ,  Annika HEINING ,  Martin HEINING ,  Michael Benjamin JOHNSON ,  Jochen LEBERT ,  Neil Francis LEININGER ,  Vinod TARWADE ,  David Allen TINSLEY

IPC: C11B1/10 ,  C11B1/04 ,  C12P7/6427

CPC classification number: C11B1/10 ,  C11B1/04 ,  C12P7/6427

Abstract: The present invention relates to a method of isolated polyunsaturated fatty acids containing lipids from a lipids containing biomass.

公开(公告)号：US20190390135A1

公开(公告)日：2019-12-26

申请号：US16563260

申请日：2019-09-06

Applicant: DSM IP ASSETS B.V.

Inventor： Neil Francis LEININGER ,  Ginger SHANK ,  Xiao DONG ,  Joseph William PFEIFER, III ,  Vidya PAI

IPC: C11B1/02 ,  C11B3/00

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

公开(公告)号：US20190338214A1

公开(公告)日：2019-11-07

申请号：US16515074

申请日：2019-07-18

Applicant: DSM IP Assets B.V.

Inventor： Stephen Robert CHERINKO ,  Robert Cody KERTIS ,  Kirk E. APT ,  Paul Warren BEHRENS ,  Jon Milton HANSEN ,  Joseph W. PFEIFER, III ,  Tracy Lynn STAHL ,  Ross E. Zirkle ,  Nasrin TABAYEHNEJAD ,  Krishna RAMAN ,  Neil Francis LEININGER

IPC: C11B1/10 ,  C11B3/16 ,  C11B3/02

Abstract: The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

公开(公告)号：US20190249108A1

公开(公告)日：2019-08-15

申请号：US16309632

申请日：2017-07-12

Applicant: Stephen Robert CHERINKO ,  Matthias DERNEDDE ,  Xiao Daniel DONG ,  Michael Benjamin JOHNSON ,  Robert Cody KERTIS ,  Jochen LEBERT ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS ,  Holger PFEIFER ,  Horst PRIEFERT ,  Christian RABE ,  Shannon Elizabeth Ethier RESOP ,  Joachim WINDAU ,  Daniel VERKOEIJEN ,  Gabriel ZAVODSKY ,  DSM IP Assets B.V. ,  Evonik Degussa GmbH

Inventor： Stephen Robert CHERINKO ,  Matthias DERNEDDE ,  Michael DIEHL ,  Xiao Daniel DONG ,  Michael Benjamin JOHNSON ,  Robert Cody KERTIS ,  Jochen LEBERT ,  Neil Francis LEININGER ,  Kirt Lyvell MATTHEWS ,  Holger PFEIFER ,  Horst PRIEFERT ,  Christian RABE ,  Shannon Elizabeth Ethier RESOP ,  Joachim WINDAU ,  Daniel VERKOEIJEN ,  Gabriel ZAVODSKY

IPC: C11B1/10 ,  C11B1/02 ,  C12N1/06 ,  C11B3/16

CPC classification number: C11B1/10 ,  A23K10/12 ,  A23K20/158 ,  C07C51/48 ,  C11B1/02 ,  C11B1/025 ,  C11B1/14 ,  C11B3/16 ,  C12N1/06 ,  C12P7/6427 ,  C12P7/6472 ,  C07C57/02

Abstract: The processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells comprise removing water from the cell fermentation broth or lysed cell composition before demulsification is conducted. Such a process has the benefits of reduced demulsification time and reduced salt use. Microbial oil comprising one or more PUFAs can be recovered from microbial cells by the process.

公开(公告)号：US20210024966A1

公开(公告)日：2021-01-28

申请号：US17042788

申请日：2019-03-29

Applicant: DSM IP Assets B.V. ,  Evonik Operations GmbH

Inventor： Martin HEINING ,  Michael Benjamin JOHNSON ,  Neil Francis LEININGER ,  Shannon Elizabeth Ethier RESOP ,  Justin Edward STANCZYK ,  Vinod TARWADE

IPC: C12P7/64

Abstract: Disclosed herein are processes for reducing emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by maintaining the level of carbohydrate at less than 15 g/Kg in the fermentation broth. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

公开(公告)号：US20150018435A1

公开(公告)日：2015-01-15

申请号：US14495406

申请日：2014-09-24

Applicant: DSM IP Assets B.V.

Inventor： Jaouad FICHTALI ,  Neil Francis LEININGER ,  Jesus Ruben ABRIL ,  Naseer AHMED ,  S. P. Janaka Namal SENANAYAKE

IPC: A23D7/04 ,  C11B15/00 ,  C11B3/00 ,  A23D9/06 ,  C11B3/08 ,  A23D7/06 ,  A23D9/04 ,  A61K47/12 ,  C11B1/10

CPC classification number: A23D7/04 ,  A23D7/06 ,  A23D9/013 ,  A23D9/04 ,  A23D9/06 ,  A61K47/12 ,  C11B1/025 ,  C11B1/10 ,  C11B3/001 ,  C11B3/008 ,  C11B3/08 ,  C11B3/10 ,  C11B5/0092 ,  C11B7/0075 ,  C11B15/00

Abstract: The present invention includes a solid fat composition that includes an oil having saturated fat and a microbial oil having a long chain polyunsaturated fatty acid and an emulsifier. In particular, the solid fat composition can have high levels of long chain polyunsaturated fatty acid and low amounts of emulsifiers. In preferred embodiments, the polyunsaturated oil is an unwinterized microbial oil. The invention also relates to methods for making such compositions and food, nutritional, and pharmaceutical products comprising said compositions. The present invention also includes a microbial oil product prepared by extracting an oil-containing fraction comprising at least one LC-PUFA from a microbial biomass, and treating the fraction by a process of vacuum evaporation, wherein the oil product has not been subject to one or more of a solvent winterization step, a caustic refining process, a chill filtration process, or a bleaching process.

Abstract translation: 本发明包括具有饱和脂肪的油和具有长链多不饱和脂肪酸和乳化剂的微生物油的固体脂肪组合物。 特别地，固体脂肪组合物可以具有高水平的长链多不饱和脂肪酸和少量的乳化剂。 在优选的实施方案中，多不饱和的油是未连续的微生物油。 本发明还涉及制备这种组合物的方法以及包含所述组合物的食品，营养和药物产品。 本发明还包括通过从微生物生物质提取包含至少一种LC-PUFA的含油馏分制备的微生物油产物，并通过真空蒸发处理该馏分，其中该油产品未被一个 或更多的溶剂冬季化步骤，苛性碱精炼方法，冷却过滤方法或漂白方法。