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公开(公告)号：US20120270204A1
公开(公告)日：2012-10-25
申请号：US13398467
申请日：2012-02-16
CPC分类号： B82Y5/00 , C12N5/0612 , H01F1/0054
摘要： A method for magnetic sorting of mammalian sperm cells having damaged membranes is described. In an embodiment of the invention, carboxyl-group functionalized magnetic particles are conjugated to propidium iodide, the resulting composition is mixed with a sample of sperm cells, and sperm cells bound to magnetic particles are separated by magnetic-activated cell sorting.
摘要翻译： 描述了具有损伤的膜的哺乳动物精细胞的磁选分选方法。 在本发明的一个实施方案中，将羧基官能化的磁性颗粒与碘化丙啶缀合，将所得组合物与精子细胞样品混合，通过磁激活细胞分选分离与磁性颗粒结合的精子细胞。
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2.发明申请公开(公告)号：US20120105967A1
公开(公告)日：2012-05-03
申请号：US13100261
申请日：2011-05-03
IPC分类号： G02B27/14
CPC分类号： G01N15/1436 , G01N15/1459 , G02B27/141
摘要： Disclosed is an optical combiner for combining multiple laser beams in a flow cytometer. A dichroic beam combiner is used to combine a second laser beam with a first laser beam so that the two beams are collinear. A beam size adjuster is utilized to adjust the size and convergence/divergence of the second laser beam so that both laser beams focus in a vertical direction at the same location on a stream in the flow cytometer. A cylindrical lens with a vertically oriented axis in the focusable beam shaping optics can also be adjusted to adjust the location of the focus point of the two beams in the horizontal direction. Alignment is maintained with the opto-mechanical adjustments made on one laser beam relative to the other laser beam path. Additional beams can also be added to the optical path.
摘要翻译： 公开了一种用于在流式细胞仪中组合多个激光束的光学组合器。 二向色光束组合器用于将第二激光束与第一激光束组合，使得两个光束共线。 利用光束尺寸调节器来调节第二激光束的尺寸和收敛/发散，使得两个激光束在流式细胞仪中的流上的相同位置处在垂直方向上聚焦。 也可以调节可聚焦光束成形光学器件中具有垂直取向轴线的柱面透镜，以调节两个光束在水平方向上的聚焦点的位置。 通过在一个激光束上相对于另一个激光束路径进行光学机械调节来维持对准。 另外的光束也可以添加到光路中。
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公开(公告)号：US20110010144A1
公开(公告)日：2011-01-13
申请号：US12683395
申请日：2010-01-06
IPC分类号： G06F17/10
CPC分类号： G01N15/1459 , G01N2015/149
摘要： Disclosed is a self-tuning flow cytometer that uses a mathematical model to perform sort decisions that is based upon the biological response of the particular types of cells that are being sorted. In one embodiment, statistical calculations of the likelihood of an event belonging to a certain population are used to make the sort decisions. Automated self-tuning processes are used to optimize the operating parameters of the flow cytometer to achieve a selected purity with higher yield at optimal sorting speeds. The fully automated processes minimize user input and allows the user to select a desired purity while maximizing yield.
摘要翻译： 公开了一种自调整流式细胞仪，其使用数学模型来执行基于正在分类的特定类型的细胞的生物反应的排序决定。 在一个实施例中，使用属于某个群体的事件的可能性的统计计算来进行排序决定。 自动自调整过程用于优化流式细胞仪的操作参数，以在最佳分选速度下以更高的产量实现选定的纯度。 完全自动化的过程最大限度地减少用户输入，并允许用户选择所需的纯度，同时最大化产量。
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公开(公告)号：US08557587B2
公开(公告)日：2013-10-15
申请号：US12683395
申请日：2010-01-06
CPC分类号： G01N15/1459 , G01N2015/149
摘要： Disclosed is a self-tuning flow cytometer that uses a mathematical model to perform sort decisions that is based upon the biological response of the particular types of cells that are being sorted. In one embodiment, statistical calculations of the likelihood of an event belonging to a certain population are used to make the sort decisions. Automated self-tuning processes are used to optimize the operating parameters of the flow cytometer to achieve a selected purity with higher yield at optimal sorting speeds. The fully automated processes minimize user input and allows the user to select a desired purity while maximizing yield.
摘要翻译： 公开了一种自调整流式细胞仪，其使用数学模型来执行基于正在分类的特定类型的细胞的生物反应的排序决定。 在一个实施例中，使用属于某个群体的事件的可能性的统计计算来进行排序决定。 自动自调整过程用于优化流式细胞仪的操作参数，以在最佳分选速度下以更高的产量实现选定的纯度。 完全自动化的过程最大限度地减少用户输入，并允许用户选择所需的纯度，同时最大化产量。
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5.发明授权公开(公告)号：US08619370B2
公开(公告)日：2013-12-31
申请号：US13100261
申请日：2011-05-03
IPC分类号： G02B27/14
CPC分类号： G01N15/1436 , G01N15/1459 , G02B27/141
摘要： Disclosed is an optical combiner for combining multiple laser beams in a flow cytometer. A dichroic beam combiner is used to combine a second laser beam with a first laser beam so that the two beams are collinear. A beam size adjuster is utilized to adjust the size and convergence/divergence of the second laser beam so that both laser beams focus in a vertical direction at the same location on a stream in the flow cytometer. A cylindrical lens with a vertically oriented axis in the focusable beam shaping optics can also be adjusted to adjust the location of the focus point of the two beams in the horizontal direction. Alignment is maintained with the opto-mechanical adjustments made on one laser beam relative to the other laser beam path. Additional beams can also be added to the optical path.
摘要翻译： 公开了一种用于在流式细胞仪中组合多个激光束的光学组合器。 二向色光束组合器用于将第二激光束与第一激光束组合，使得两个光束共线。 利用光束尺寸调节器来调节第二激光束的尺寸和收敛/发散，使得两个激光束在流式细胞仪中的流上的相同位置处在垂直方向上聚焦。 也可以调节可聚焦光束成形光学器件中具有垂直取向轴线的柱面透镜，以调节两个光束在水平方向上的聚焦点的位置。 通过在一个激光束上相对于另一个激光束路径进行光学机械调节来维持对准。 另外的光束也可以添加到光路中。
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