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    Method of cryopreserving selected sperm cells
    2.
    发明授权
    Method of cryopreserving selected sperm cells 有权
    冷冻保存精选细胞的方法

    公开(公告)号：US07820425B2

    公开(公告)日：2010-10-26

    申请号：US11608079

    申请日：2006-12-07

    申请人： John L. Schenk

    发明人： John L. Schenk

    IPC分类号： C12N9/24

    CPC分类号： A01N1/021 ,  A01N1/02 ,  A01N1/0215 ,  A01N1/0221 ,  A01N1/0226

    摘要： The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

    摘要翻译： 本发明提供了针对特定特征选择的冷冻保存精子的方法。 在优选的实施方案中，使用该方法来冷冻性别选择的精子。 尽管本发明的冷冻保存方法可用于冷冻通过任何数量的选择方法选择的精子，但优选使用流式细胞术进行选择。 本发明还提供了针对特定特征例如性别类型选择的冷冻精子样品。 在优选实施方案中，冷冻精子样品包括哺乳动物精子，例如人，牛，马，猪，羊，麋鹿或野牛精子。 冷冻选择的精子样品可用于各种应用。 特别地，样品可以解冻并用于受精。 因此，本发明还包括使用冷冻选择的精子样品进行人工授精或体外受精的方法。

    Method of cryopreserving selected sperm cells
    5.
    发明授权
    Method of cryopreserving selected sperm cells 有权
    冷冻保存精选细胞的方法

    公开(公告)号：US07208265B1

    公开(公告)日：2007-04-24

    申请号：US09478299

    申请日：2000-01-05

    申请人： John L. Schenk

    发明人： John L. Schenk

    IPC分类号： A01N1/00

    CPC分类号： A01N1/021 ,  A01N1/02 ,  A01N1/0215 ,  A01N1/0221 ,  A01N1/0226

    摘要： The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

    摘要翻译： 本发明提供了针对特定特征选择的冷冻保存精子的方法。 在优选的实施方案中，使用该方法来冷冻性别选择的精子。 尽管本发明的冷冻保存方法可用于冷冻通过任何数量的选择方法选择的精子，但优选使用流式细胞术进行选择。 本发明还提供了针对特定特征例如性别类型选择的冷冻精子样品。 在优选实施方案中，冷冻精子样品包括哺乳动物精子，例如人，牛，马，猪，羊，麋鹿或野牛精子。 冷冻选择的精子样品可用于各种应用。 特别地，样品可以解冻并用于受精。 因此，本发明还包括使用冷冻选择的精子样品进行人工授精或体外受精的方法。

    Methods and apparatus for reducing protein content in sperm cell extenders
    6.
    发明授权
    Methods and apparatus for reducing protein content in sperm cell extenders 有权

    公开(公告)号：US08426197B2

    公开(公告)日：2013-04-23

    申请号：US12619586

    申请日：2009-11-16

    申请人： John L. Schenk

    发明人： John L. Schenk

    IPC分类号： A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.

    Cryopreserved selected sperm cell sample
    7.
    发明申请
    Cryopreserved selected sperm cell sample 审中-公开
    冷冻保存精选细胞样品

    公开(公告)号：US20110004052A1

    公开(公告)日：2011-01-06

    申请号：US12807555

    申请日：2010-09-07

    申请人： John L. Schenk

    发明人： John L. Schenk

    IPC分类号： A61B17/43 ,  C12N5/076 ,  A61D19/02

    CPC分类号： A01N1/021 ,  A01N1/02 ,  A01N1/0215 ,  A01N1/0221 ,  A01N1/0226

    摘要： The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

    摘要翻译： 本发明提供了针对特定特征选择的冷冻保存精子的方法。 在优选的实施方案中，使用该方法来冷冻性别选择的精子。 尽管本发明的冷冻保存方法可用于冷冻通过任何数量的选择方法选择的精子，但优选使用流式细胞术进行选择。 本发明还提供了针对特定特征例如性别类型选择的冷冻精子样品。 在优选实施方案中，冷冻精子样品包括哺乳动物精子，例如人，牛，马，猪，羊，麋鹿或野牛精子。 冷冻选择的精子样品可用于各种应用。 特别地，样品可以解冻并用于受精。 因此，本发明还包括使用冷冻选择的精子样品进行人工授精或体外受精的方法。

    Methods and apparatus for reducing protein content in sperm cell extenders
    8.
    发明授权
    Methods and apparatus for reducing protein content in sperm cell extenders 有权
    降低精子细胞增殖物蛋白质含量的方法和装置

    公开(公告)号：US07618770B2

    公开(公告)日：2009-11-17

    申请号：US11219607

    申请日：2005-09-02

    申请人： John L. Schenk

    发明人： John L. Schenk

    IPC分类号： A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.

    摘要翻译： 本发明的技术涉及用于减少精子细胞扩增剂中的蛋白质含量的方法和装置，并且可以包括以下特征中的一个或多个：用于降低精子细胞增量剂中的蛋白质含量的技术; 用于降低含有冷冻保护剂的B部分精子细胞延长剂中的蛋白质含量的技术; 制备不需要澄清的精子细胞扩增剂的技术; 制备适合离心的低密度梯度精子细胞增量剂的技术; 用于在制备精子细胞延长剂中的各个步骤之间降低蛋白质含量的技术以及用于在精子细胞扩增体中建立降低的蛋白质含量的新值的技术。