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1.发明授权公开(公告)号：US08619370B2
公开(公告)日：2013-12-31
申请号：US13100261
申请日：2011-05-03
IPC分类号： G02B27/14
CPC分类号： G01N15/1436 , G01N15/1459 , G02B27/141
摘要： Disclosed is an optical combiner for combining multiple laser beams in a flow cytometer. A dichroic beam combiner is used to combine a second laser beam with a first laser beam so that the two beams are collinear. A beam size adjuster is utilized to adjust the size and convergence/divergence of the second laser beam so that both laser beams focus in a vertical direction at the same location on a stream in the flow cytometer. A cylindrical lens with a vertically oriented axis in the focusable beam shaping optics can also be adjusted to adjust the location of the focus point of the two beams in the horizontal direction. Alignment is maintained with the opto-mechanical adjustments made on one laser beam relative to the other laser beam path. Additional beams can also be added to the optical path.
摘要翻译： 公开了一种用于在流式细胞仪中组合多个激光束的光学组合器。 二向色光束组合器用于将第二激光束与第一激光束组合，使得两个光束共线。 利用光束尺寸调节器来调节第二激光束的尺寸和收敛/发散，使得两个激光束在流式细胞仪中的流上的相同位置处在垂直方向上聚焦。 也可以调节可聚焦光束成形光学器件中具有垂直取向轴线的柱面透镜，以调节两个光束在水平方向上的聚焦点的位置。 通过在一个激光束上相对于另一个激光束路径进行光学机械调节来维持对准。 另外的光束也可以添加到光路中。
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2.发明申请公开(公告)号：US20120105967A1
公开(公告)日：2012-05-03
申请号：US13100261
申请日：2011-05-03
IPC分类号： G02B27/14
CPC分类号： G01N15/1436 , G01N15/1459 , G02B27/141
摘要： Disclosed is an optical combiner for combining multiple laser beams in a flow cytometer. A dichroic beam combiner is used to combine a second laser beam with a first laser beam so that the two beams are collinear. A beam size adjuster is utilized to adjust the size and convergence/divergence of the second laser beam so that both laser beams focus in a vertical direction at the same location on a stream in the flow cytometer. A cylindrical lens with a vertically oriented axis in the focusable beam shaping optics can also be adjusted to adjust the location of the focus point of the two beams in the horizontal direction. Alignment is maintained with the opto-mechanical adjustments made on one laser beam relative to the other laser beam path. Additional beams can also be added to the optical path.
摘要翻译： 公开了一种用于在流式细胞仪中组合多个激光束的光学组合器。 二向色光束组合器用于将第二激光束与第一激光束组合，使得两个光束共线。 利用光束尺寸调节器来调节第二激光束的尺寸和收敛/发散，使得两个激光束在流式细胞仪中的流上的相同位置处在垂直方向上聚焦。 也可以调节可聚焦光束成形光学器件中具有垂直取向轴线的柱面透镜，以调节两个光束在水平方向上的聚焦点的位置。 通过在一个激光束上相对于另一个激光束路径进行光学机械调节来维持对准。 另外的光束也可以添加到光路中。
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公开(公告)号：US08557587B2
公开(公告)日：2013-10-15
申请号：US12683395
申请日：2010-01-06
CPC分类号： G01N15/1459 , G01N2015/149
摘要： Disclosed is a self-tuning flow cytometer that uses a mathematical model to perform sort decisions that is based upon the biological response of the particular types of cells that are being sorted. In one embodiment, statistical calculations of the likelihood of an event belonging to a certain population are used to make the sort decisions. Automated self-tuning processes are used to optimize the operating parameters of the flow cytometer to achieve a selected purity with higher yield at optimal sorting speeds. The fully automated processes minimize user input and allows the user to select a desired purity while maximizing yield.
摘要翻译： 公开了一种自调整流式细胞仪，其使用数学模型来执行基于正在分类的特定类型的细胞的生物反应的排序决定。 在一个实施例中，使用属于某个群体的事件的可能性的统计计算来进行排序决定。 自动自调整过程用于优化流式细胞仪的操作参数，以在最佳分选速度下以更高的产量实现选定的纯度。 完全自动化的过程最大限度地减少用户输入，并允许用户选择所需的纯度，同时最大化产量。
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公开(公告)号：US20120270204A1
公开(公告)日：2012-10-25
申请号：US13398467
申请日：2012-02-16
CPC分类号： B82Y5/00 , C12N5/0612 , H01F1/0054
摘要： A method for magnetic sorting of mammalian sperm cells having damaged membranes is described. In an embodiment of the invention, carboxyl-group functionalized magnetic particles are conjugated to propidium iodide, the resulting composition is mixed with a sample of sperm cells, and sperm cells bound to magnetic particles are separated by magnetic-activated cell sorting.
摘要翻译： 描述了具有损伤的膜的哺乳动物精细胞的磁选分选方法。 在本发明的一个实施方案中，将羧基官能化的磁性颗粒与碘化丙啶缀合，将所得组合物与精子细胞样品混合，通过磁激活细胞分选分离与磁性颗粒结合的精子细胞。
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公开(公告)号：US20110010144A1
公开(公告)日：2011-01-13
申请号：US12683395
申请日：2010-01-06
IPC分类号： G06F17/10
CPC分类号： G01N15/1459 , G01N2015/149
摘要： Disclosed is a self-tuning flow cytometer that uses a mathematical model to perform sort decisions that is based upon the biological response of the particular types of cells that are being sorted. In one embodiment, statistical calculations of the likelihood of an event belonging to a certain population are used to make the sort decisions. Automated self-tuning processes are used to optimize the operating parameters of the flow cytometer to achieve a selected purity with higher yield at optimal sorting speeds. The fully automated processes minimize user input and allows the user to select a desired purity while maximizing yield.
摘要翻译： 公开了一种自调整流式细胞仪，其使用数学模型来执行基于正在分类的特定类型的细胞的生物反应的排序决定。 在一个实施例中，使用属于某个群体的事件的可能性的统计计算来进行排序决定。 自动自调整过程用于优化流式细胞仪的操作参数，以在最佳分选速度下以更高的产量实现选定的纯度。 完全自动化的过程最大限度地减少用户输入，并允许用户选择所需的纯度，同时最大化产量。
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公开(公告)号：US20110303859A1
公开(公告)日：2011-12-15
申请号：US13044685
申请日：2011-03-10
CPC分类号： G01N15/1429 , G01N15/1427 , G01N15/1459 , G01N2015/1438
摘要： A method is provided for generating measurement parameters for a particle sample in a particle analyzer. The method includes, interrogating the particle sample with a triggering interrogator and one or more secondary interrogators respectively positioned along a length of an interrogation area, generating respective pulses based upon the interrogation of a first particle from the particle sample, determining a primary pulse detection window based upon a triggering pulse, determining a search interval to find a secondary pulse based upon factors including the primary pulse detection window and a laser delay, adjusting the search interval for laser delay variation dynamically based on the interrogation of the first particle, identifying the secondary pulse in the adjusted search interval, and processing the secondary pulse to determine a peak value of the secondary pulse. Corresponding apparatus are also provided.
摘要翻译： 提供了一种用于在粒子分析仪中产生粒子样本的测量参数的方法。 该方法包括：用触发询问器和一个或多个二级询问器询问粒子样本，该询问器分别沿着询问区域的长度定位，根据来自粒子样本的第一粒子的询问产生相应的脉冲，确定主脉冲检测窗口 基于触发脉冲，基于包括主脉冲检测窗口和激光延迟的因素来确定搜索间隔以找到辅助脉冲，基于第一颗粒的询问来动态地调整激光延迟变化的搜索间隔，识别次级 脉冲在调整的搜索间隔中，并且处理辅助脉冲以确定次级脉冲的峰值。 还提供了相应的装置。
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7.发明申请MULTI-GAIN ADAPTIVE LINEAR PROCESSING AND GATED DIGITAL SYSTEM FOR USE IN FLOW CYTOMETRY 有权多增益自适应线性处理和流动细胞计数中使用的数字系统公开(公告)号：US20080319680A1
公开(公告)日：2008-12-25
申请号：US12053439
申请日：2008-03-21
申请人： Daniel N. Fox , Thomas L. Thrasher
发明人： Daniel N. Fox , Thomas L. Thrasher
CPC分类号： G01N15/147 , G01N15/1429 , G01N2015/1477 , H04B17/0082 , H04B17/0085
摘要： Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.
摘要翻译： 公开了一种用于流式细胞仪的电子处理系统，其使用处理芯片，其以逐个样本的形式处理并行架构中的数据，并提供高吞吐量的数据。 此外，使用多增益线性放大器，其使用反馈电路匹配以提供具有高动态范围的精确数据和高分辨率数据。
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8.发明授权Multi-gain adaptive linear processing and gated digital system for use in flow cytometry 有权多增益自适应线性处理和门控数字系统用于流式细胞术公开(公告)号：US09243995B2
公开(公告)日：2016-01-26
申请号：US13619845
申请日：2012-09-14
申请人： Daniel N. Fox , Thomas L. Thrasher
发明人： Daniel N. Fox , Thomas L. Thrasher
CPC分类号： G01N15/147 , G01N15/1429 , G01N2015/1477 , H04B17/0082 , H04B17/0085
摘要： Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.
摘要翻译： 公开了一种用于流式细胞仪的电子处理系统，其使用处理芯片，其以逐个样本的形式处理并行架构中的数据，并提供高吞吐量的数据。 此外，使用多增益线性放大器，其使用反馈电路匹配以提供具有高动态范围的精确数据和高分辨率数据。
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9.发明申请Multi-gain Adaptive Linear Processing and Gated Digital System for Use in Flow Cytometry 有权用于流式细胞术的多增益自适应线性处理和门控数字系统公开(公告)号：US20130013226A1
公开(公告)日：2013-01-10
申请号：US13619845
申请日：2012-09-14
申请人： Daniel N. Fox , Thomas L. Thrasher
发明人： Daniel N. Fox , Thomas L. Thrasher
CPC分类号： G01N15/147 , G01N15/1429 , G01N2015/1477 , H04B17/0082 , H04B17/0085
摘要： Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.
摘要翻译： 公开了一种用于流式细胞仪的电子处理系统，其使用处理芯片，其以逐个样本的形式处理并行架构中的数据，并提供高吞吐量的数据。 此外，使用多增益线性放大器，其使用反馈电路匹配以提供具有高动态范围的精确数据和高分辨率数据。
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公开(公告)号：US07590500B2
公开(公告)日：2009-09-15
申请号：US10587887
申请日：2005-02-28
IPC分类号： G01D18/00
CPC分类号： G01N15/1459 , G01N15/1429 , G01N2015/149
摘要： A flow cytometer capable of using electronics to analyze a large dynamic range while avoiding and/or correcting traditional limitations of acquisition electronics. The invention allows for measuring signal characteristics, which may include but are not limited to linear peak, integral, logarithmic peak, and pulse width. Other system parameters may also be generated by the invention to indicate timing or status information. Accurate and precise measurements may be performed by correcting for input errors if needed, and by transforming the input signal to produce a more easily measured form.
摘要翻译： 流式细胞仪能够使用电子学来分析大的动态范围，同时避免和/或纠正采集电子的传统限制。 本发明允许测量信号特性，其可以包括但不限于线性峰值，积分，对数峰值和脉冲宽度。 本发明也可以产生其他系统参数以指示定时或状态信息。 可以通过根据需要校正输入误差，并且通过变换输入信号以产生更容易测量的形式来执行准确和精确的测量。
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