公开(公告)号：US20150152458A1

公开(公告)日：2015-06-04

申请号：US14479653

申请日：2013-03-26

Applicant: Danisco US Inc.

Inventor： Bart Christian Koops ,  Floor K. Kooy ,  Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Arjen Hendrik van Tuijl

IPC: C12P19/20

CPC classification number: C12P19/20 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01003 ,  C13K1/06

Abstract: The present teachings provide a method for making a high glucose syrup at a low temperature. In some embodiments, the syrup contains reduced reversion reaction products. The method comprises contacting a starch substrate at a temperature below the starch gelatinization temperature with an enzyme blend comprising a high dose of alpha-amylase and a low dose of glucoamylase. In some embodiments, a blend of two glucoamylases is employed. In some embodiments, a debranching enzyme such as a pullulanase is employed. In some embodiments, the enzymes are staged by adding at different times, or at different temperatures. The present teachings provide for high glucose syrups with fewer reversion products, and allow for higher starch solubilization.

Abstract translation: 本教导提供了一种在低温下制备高糖浆的方法。 在一些实施方案中，糖浆含有降低的逆转反应产物。 该方法包括在低于淀粉糊化温度的温度下将淀粉底物与包含高剂量的α-淀粉酶和低剂量葡糖淀粉酶的酶混合物接触。 在一些实施方案中，使用两种葡糖淀粉酶的共混物。 在一些实施方案中，使用脱支酶，例如支链淀粉酶。 在一些实施方案中，通过在不同时间或在不同的温度下加入来分级酶。 本教导提供具有较少逆转产物的高葡萄糖浆，并允许更高的淀粉溶解。

公开(公告)号：US20150152457A1

公开(公告)日：2015-06-04

申请号：US14479683

申请日：2013-03-13

Applicant: Danisco US Inc.

Inventor： Collette Michelle Blake ,  Gang Duan ,  Douglas Ko ,  Floor K. Kooy ,  Sung Ho Lee ,  Michael Jay Pepsin ,  Ying Qian ,  Matthew T. Reboli ,  Vivek Sharma ,  Jayarama K. Shetty ,  Bruce A. Strohm ,  Paula Johanna Maria Teunissen ,  Hongxian Xu

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P19/02 ,  C12P19/12 ,  C12P19/20 ,  C12Y301/03 ,  C12Y302/01001 ,  C12Y302/01003

Abstract: Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Abstract translation: 本文提供的是与将研磨或分馏的谷粒中的淀粉直接转化成可发酵糖原料相关的组合物和方法，其可以作为发酵生物体工业生产一种或多种产品的碳源。 这样的转化可以在等于或低于晶粒中存在的淀粉的初始糊化温度的温度下进行，并且可以利用存在于某些未精制谷物中的一种或多种可分离的内源性酶。

公开(公告)号：US20150111259A1

公开(公告)日：2015-04-23

申请号：US14387779

申请日：2013-03-12

Applicant: Danisco US Inc.

Inventor： T. Kleinhout ,  Bart Christiaan Koops ,  Floor K. Kooy ,  Sung Ho Lee ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12P19/12 ,  C12P19/14

CPC classification number: C12P19/12 ,  A23L29/35 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01002 ,  C12Y302/01068 ,  C12Y302/01133 ,  C13K7/00

Abstract: The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same.

Abstract translation: 本教导提供将颗粒淀粉直接转化成包含高，非常高和/或超高麦芽糖含量的可溶性糖组合物。 该方法包括使颗粒状淀粉的含水浆液与包含α-淀粉酶和麦芽糖酶的合适比例的酶组合物接触，以产生酶促转化为含有较高麦芽糖的糖组合物的可溶性淀粉底物。 该过程可以使用多于一个温度来实现。