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    Polynucleotides encoding anti-sulfotyrosine antibodies
    7.
    发明授权
    Polynucleotides encoding anti-sulfotyrosine antibodies 有权
    编码抗硫代酪氨酸抗体的多核苷酸

    公开(公告)号：US07868152B2

    公开(公告)日：2011-01-11

    申请号：US12560294

    申请日：2009-09-15

    申请人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    发明人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N5/07 ,  C12N5/16

    CPC分类号： C07K16/44 ,  C07K2317/56 ,  C07K2317/622

    摘要： The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

    摘要翻译： 本发明提供能够检测和分离酪氨酸硫酸化的多肽的抗硫代酪氨酸特异性抗体。 本发明的硫代酪氨酸抗体和抗体片段可用于使用任何数量的免疫学测定法，例如ELISA，免疫印迹，Western印迹，免疫沉淀等来区分这些蛋白质的非硫酸化和硫酸化形式。 使用噬菌体展示系统，产生单链抗体（scFv）并筛选抗酪氨酸硫酸化的合成肽抗原，导致分离特异性识别含磺酰酪氨酸的肽和/或在酪氨酸硫酸化蛋白中显示磺酰酪氨酸特异性结合的scFv 。 使用来自一种这样的磺基酪氨酸特异性scFv的VH和VL基因产生全长磺酰酪氨酸特异性免疫球蛋白。

    POLYNUCLEOTIDES ENCODING ANTI-SULFOTYROSINE ANTIBODIES
    8.
    发明申请
    POLYNUCLEOTIDES ENCODING ANTI-SULFOTYROSINE ANTIBODIES 有权
    编码抗硫霉素抗体的多核苷酸

    公开(公告)号：US20100009441A1

    公开(公告)日：2010-01-14

    申请号：US12560294

    申请日：2009-09-15

    申请人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    发明人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    IPC分类号： C12N5/06 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C07K16/44 ,  C07K2317/56 ,  C07K2317/622

    摘要： The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

    摘要翻译： 本发明提供能够检测和分离酪氨酸硫酸化的多肽的抗硫代酪氨酸特异性抗体。 本发明的硫代酪氨酸抗体和抗体片段可用于使用任何数量的免疫学测定法，例如ELISA，免疫印迹，Western印迹，免疫沉淀等来区分这些蛋白质的非硫酸化和硫酸化形式。 使用噬菌体展示系统，产生单链抗体（scFv）并筛选抗酪氨酸硫酸化的合成肽抗原，导致分离特异性识别含磺酰酪氨酸的肽和/或在酪氨酸硫酸化蛋白中显示磺酰酪氨酸特异性结合的scFv 。 使用来自一种这样的磺基酪氨酸特异性scFv的VH和VL基因产生全长磺酰酪氨酸特异性免疫球蛋白。

    Anti-sulfotyrosine antibodies
    9.
    发明授权
    Anti-sulfotyrosine antibodies 有权
    抗硫代酪氨酸抗体

    公开(公告)号：US07589182B1

    公开(公告)日：2009-09-15

    申请号：US11900689

    申请日：2007-09-12

    申请人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    发明人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    IPC分类号： C07K16/00 ,  C07K16/46 ,  A61K39/395

    CPC分类号： C07K16/44 ,  C07K2317/56 ,  C07K2317/622

    摘要： The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

    摘要翻译： 本发明提供能够检测和分离酪氨酸硫酸化的多肽的抗硫代酪氨酸特异性抗体。 本发明的硫代酪氨酸抗体和抗体片段可用于使用任何数量的免疫学测定法，例如ELISA，免疫印迹，Western印迹，免疫沉淀等来区分这些蛋白质的非硫酸化和硫酸化形式。 使用噬菌体展示系统，产生单链抗体（scFv）并筛选抗酪氨酸硫酸化的合成肽抗原，导致分离特异性识别含磺酰酪氨酸的肽和/或在酪氨酸硫酸化蛋白中显示磺酰酪氨酸特异性结合的scFv 。 使用来自一种这样的磺基酪氨酸特异性scFv的VH和VL基因产生全长磺酰酪氨酸特异性免疫球蛋白。

    EGFR binding peptides and uses thereof
    10.
    发明授权
    EGFR binding peptides and uses thereof 有权
    EGFR结合肽及其用途

    公开(公告)号：US08536113B2

    公开(公告)日：2013-09-17

    申请号：US12514622

    申请日：2007-12-20

    申请人： Mohit Trikha ,  Wang Bryan ,  Frank Shi ,  John Kehoe

    发明人： Mohit Trikha ,  Wang Bryan ,  Frank Shi ,  John Kehoe

    IPC分类号： A61K9/127

    CPC分类号： C07K7/08 ,  A61K38/00 ,  C07K7/06

    摘要： Short oligopeptides are provided which are capable of binding to the epidermal growth factor receptor on the surface of human tumor cells. Methods for using the peptides as targeting moieties in more complex compositions, such as conjugates of cytoxins, and/or structures, such as liposomal structures, for the purposes of drug delivery are also provided.

    摘要翻译： 提供能够结合人肿瘤细胞表面上的表皮生长因子受体的短寡肽。 还提供了用于在更复杂的组合物中使用肽作为靶向部分的方法，例如用于药物递送目的的细胞毒素和/或结构（例如脂质体结构）的缀合物。