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公开(公告)号：US4827079A
公开(公告)日：1989-05-02
申请号：US738738
申请日：1985-07-03
CPC分类号： C12N5/0025 , A01H1/02 , A01H4/005 , A01H9/00 , C12N5/04
摘要： A method is presented which permits the induction and screening of variant plants which display a Non-Mendelian mode of inheritance. The process involves maintaining plant tissue in culture for a limited period of time in the presence of unusually high levels of growth regulators and screening regenerated plants recovered therefrom. Novel varities resulting from the process of the invention are described.
摘要翻译： 提出了一种方法，其允许诱导和筛选显示非孟德尔遗传模式的变体植物。 该过程包括在存在异常高水平的生长调节剂并筛选从其回收的再生植物的情况下将培养物中的植物组织维持在有限的时间段内。 描述由本发明的方法产生的新型变体。
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公开(公告)号：US4818699A
公开(公告)日：1989-04-04
申请号：US525092
申请日：1983-08-22
CPC分类号： C12N5/04 , A01H1/02 , A01H4/005 , A01H9/00 , C12N5/0025
摘要： A method is presented which permits the induction and screening of variant plants which display a Non-Mendelian mode of inheritance. The process involves maintaining plant tissue in culture for a limited period of time in the presence of unusually high levels of growth regulators and screening regenerated plants recovered therefrom.
摘要翻译： 提出了一种方法，其允许诱导和筛选显示非孟德尔遗传模式的变体植物。 该过程包括在存在异常高水平的生长调节剂并筛选从其回收的再生植物的情况下将培养物中的植物组织维持在有限的时间段内。
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3.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US06803499B1
公开(公告)日：2004-10-12
申请号：US08852340
申请日：1997-05-07
申请人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
发明人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
IPC分类号： C12N504
CPC分类号： A01H4/001 , A01H4/00 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N15/8207 , C12N15/8241 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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4.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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5.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US06399861B1
公开(公告)日：2002-06-04
申请号：US08447985
申请日：1995-05-23
申请人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
发明人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
IPC分类号： A01H500
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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