公开(公告)号：US08951527B2

公开(公告)日：2015-02-10

申请号：US13057447

申请日：2009-08-05

Applicant: Jeffrey S. Isenberg ,  David D. Roberts ,  Justin Maxhimer

Inventor： Jeffrey S. Isenberg ,  David D. Roberts ,  Justin Maxhimer

IPC: A61K38/08 ,  A61K38/10 ,  A61K38/36 ,  C12N15/11 ,  A61K38/39 ,  C07K14/705 ,  C07K14/78 ,  C07K16/18 ,  C12N15/113 ,  C07K16/28 ,  A61K39/00 ,  A61N5/10

CPC classification number: A61K38/39 ,  A61K2039/505 ,  A61N2005/1094 ,  C07K14/70596 ,  C07K14/78 ,  C07K16/18 ,  C07K16/2803 ,  C07K2317/70 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/3233

Abstract: Described herein is the discovery that cell and tissue survival can be dramatically increased following radiation exposure through inhibition of the interaction between TSP-1 and CD47. This effect is shown using antisense molecules, peptides, and antibodies, which can now be used as radioprotectant agents. These agents find application in minimizing, reducing and/or preventing tissue damage following intentional and accidental radiation exposure, as well as increasing the therapeutic efficacy of radiation therapies by protecting non-target tissue from incidental radiation damage and by increasing tumor ablation following radiation treatment.

Abstract translation: 本文描述的是通过抑制TSP-1和CD47之间的相互作用在辐射暴露后细胞和组织存活可以显着增加的发现。 这种效应使用反义分子，肽和抗体显示，其现在可以用作放射防护剂。 这些试剂在减少，减少和/或预防有意和意外的辐射暴露后的组织损伤中的应用，以及通过保护非目标组织免受附带的辐射损伤并通过在放射治疗之后增加肿瘤消融来增加放射疗法的治疗功效。

公开(公告)号：US20080249026A1

公开(公告)日：2008-10-09

申请号：US11466718

申请日：2006-08-23

Applicant: David D. Roberts ,  Henry C. Krutzsch

Inventor： David D. Roberts ,  Henry C. Krutzsch

IPC: A61K38/10 ,  C07K5/00 ,  C07K7/06 ,  C07K7/08 ,  A61P35/00 ,  C12N5/00 ,  A61P9/00 ,  A61K38/07 ,  A61K38/08

CPC classification number: C07K7/06 ,  A61K38/00

Abstract: The present invention relates to a peptide comprising the sequence R1-X1-X2-X3-X4-R2, wherein X1 is selected from the group consisting of N, Q, D and S; X2 is selected from the group consisting of V, I and L; X1 is selected from the group consisting of R and K; and X4 is selected from the group consisting of V, I, L and F; R1 is a hydrogen or a peptide of 1 to 6 amino acids, and acyl or an aryl group; and R2 is a peptide of 1 to 3 amino acids, a hydroxide or an amide. The invention also relates to partial or full retro-inverso peptides comprising the above sequences. The invention also relates to peptide-substrate combination comprising a substrate suitable for cell growth and the peptide of the invention, and to a vascular graft and an artificial blood vessel comprising the peptide-substrate combination. The invention also relates to a pharmaceutical composition and a peptide conjugate comprising the peptide of the invention. The invention also relates to a method of inhibiting adhesion of a cell expressing α3β1 integrin to an extracellular matrix, inhibiting α3β1-integrin-mediated cell motility, inhibiting α3β1-integrin mediated cell proliferation, promoting α3β1-integrin mediated cell proliferation and inhibiting angiogenesis utilizing the peptides of the invention.

Abstract translation: 本发明涉及包含序列R 1 -X 1 -X 2 -X 3 -X 3的肽， X 4 -R 2，其中X 1选自N，Q，D和S; X 2选自V，I和L; X 1选自R和K; 并且X 4选自V，I，L和F; R 1是氢或1至6个氨基酸的肽，酰基或芳基; R 2是1至3个氨基酸的肽，氢氧化物或酰胺。 本发明还涉及包含上述序列的部分或全部逆转肽。 本发明还涉及包含适合于细胞生长的底物和本发明的肽的肽 - 底物组合以及包含肽 - 底物组合的血管移植物和人造血管。 本发明还涉及包含本发明的肽的药物组合物和肽缀合物。 本发明还涉及一种抑制细胞外基质表达α3β1整联蛋白的细胞粘附的方法，抑制α3β1， 整合素介导的细胞运动，抑制α3-β1整联蛋白介导的细胞增殖，促进α3β - 整合素介导的细胞增殖并利用本发明的肽抑制血管生成。

公开(公告)号：US07095585B2

公开(公告)日：2006-08-22

申请号：US10970782

申请日：2004-10-20

Applicant: Alexander P. Payne ,  William C. Cain ,  Michael E. Devillier ,  Harold J. Hamilton ,  Robert D. Hempstead ,  Darren T. Imai ,  Dimitre A. Latev ,  David D. Roberts

Inventor： Alexander P. Payne ,  William C. Cain ,  Michael E. Devillier ,  Harold J. Hamilton ,  Robert D. Hempstead ,  Darren T. Imai ,  Dimitre A. Latev ,  David D. Roberts

IPC: G11B5/147 ,  G11B5/235

CPC classification number: G11B5/6005 ,  G11B5/1278 ,  G11B5/3106 ,  G11B5/3176 ,  G11B5/3183 ,  G11B5/3967 ,  G11B5/6076

Abstract: An information storage system includes a transducer having a loop of ferromagnetic material with pole tips separated by an nonferromagnetic gap located adjacent to a medium such as a rigid disk. During writing the separation between the pole tips and the media layer of the disk is a small fraction of the gap separation. Due to the small separation between the pole tips and the media layer, the magnetic field generated by the transducer and felt by the media has a larger perpendicular than longitudinal component, favoring perpendicular recording over longitudinal recording. The media may have an easy axis of magnetization oriented substantially along the perpendicular direction, so that perpendicular data storage is energetically favored. The transducer may also include a magnetoresistive sensor for reading magnetic information from the disk.

公开(公告)号：US06320725B1

公开(公告)日：2001-11-20

申请号：US08577493

申请日：1995-12-22

Applicant: Alexander P. Payne ,  William C. Cain ,  Michael E. DeVillier ,  Harold J. Hamilton ,  Robert D. Hempstead ,  Darren T. Imai ,  Mark A. Lauer ,  Dimitre A. Latev ,  David D. Roberts

Inventor： Alexander P. Payne ,  William C. Cain ,  Michael E. DeVillier ,  Harold J. Hamilton ,  Robert D. Hempstead ,  Darren T. Imai ,  Mark A. Lauer ,  Dimitre A. Latev ,  David D. Roberts

IPC: G11B5127

CPC classification number: G11B5/6005 ,  G11B5/1278 ,  G11B5/3106 ,  G11B5/3176 ,  G11B5/3183 ,  G11B5/3967 ,  G11B5/6076

Abstract: An information storage system having a ring head sliding on a rigid magnetic storage disk in such close proximity that the magnetic field felt by the media layer or layers of the disk has a larger perpendicular than longitudinal component so that data is stored in a perpendicular mode. The head to media separation during writing of data to the media is a small fraction of the amagnetic gap separating the poletips of the head. Reading of data may be inductive or may be via a magnetoresistive sensor which is coupled to the magnetically permeable core of the ring head far from the poletips. The media preferably has a high perpendicular anisotropy.

Abstract translation: 一种信息存储系统，其具有在刚性磁存储盘上滑动的环形头，所述环形头在所述介质层或所述盘的层中感觉到的磁场具有比纵向分量更大的垂直于垂直模式的数据。 在向媒体写入数据期间的媒体分离的头部是分离头部poletips的磁性间隙的一小部分。 数据的读取可以是感性的，或者可以是经由磁阻传感器，该磁阻传感器耦合到远离耳环的环形头的导磁芯。 介质优选具有高垂直各向异性。

公开(公告)号：US5491130A

公开(公告)日：1996-02-13

申请号：US973235

申请日：1992-11-10

Applicant: David D. Roberts ,  Henry C. Krutzsch ,  John M. Sipes ,  Neng-hua Guo ,  Eric Negre

Inventor： David D. Roberts ,  Henry C. Krutzsch ,  John M. Sipes ,  Neng-hua Guo ,  Eric Negre

IPC: A61K38/00 ,  A61P43/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/47 ,  C07K14/78 ,  C07K7/00

CPC classification number: C07K14/78 ,  A61K38/00

Abstract: Peptides derived from the second type 1 repeat of human endothelial cell thrombospondin which bind to the gelatin-binding domain of fibronectin have been isolated and synthetically produced. The peptides can be used to bind to fibronectin or other related collagen-binding proteins to inhibit fibronectin-dependent cell adhesion to collagen matrices and to inhibit interactions with collagen of other proteins that share homologies with the gelatin-binding domain of fibronectin.

Abstract translation: 衍生自结合纤连蛋白的明胶结合结构域的人内皮细胞血小板反应蛋白的第二类型1重复的肽已被分离并合成产生。 肽可以用于结合纤连蛋白或其他相关的胶原结合蛋白，以抑制纤连蛋白依赖性细胞对胶原基质的粘附，并抑制与纤连蛋白的明胶结合结构域共享的其它蛋白质的胶原的相互作用。

公开(公告)号：US5225330A

公开(公告)日：1993-07-06

申请号：US417691

申请日：1989-10-05

Applicant: Victor Ginsburg ,  Howard C. Krivan ,  David D. Roberts

Inventor： Victor Ginsburg ,  Howard C. Krivan ,  David D. Roberts

IPC: C12Q1/04 ,  A61K31/70 ,  C07H15/00 ,  C12N11/00 ,  C12Q1/24 ,  C12R1/01 ,  C12R1/19 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/569

CPC classification number: G01N33/56933 ,  A61K31/70 ,  A61K31/7004 ,  C12Q1/24 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/566 ,  G01N33/569 ,  G01N33/56911 ,  G01N2333/30 ,  G01N2400/00 ,  Y10S435/87 ,  Y10S435/975

Abstract: A diagnostic kit for detecting the presence of microorganisms, comprising an insoluble substrate; and a carbohydrate receptor immobilized on the insoluble substrate, the carbohydrate receptor being capable of adsorbing microorganisms; and a labelled reagent useful for detecting the presence of microorganisms bound to the carbohydrate receptors and a method for detecting the presence of specified microorganisms in a sample, which comprises contacting a sample to be tested with carbohydrate receptors immobilized on an insoluble substrate; and determining the extent of binding of microorganisms in the sample to the carbohydrate receptors by use of a labelled reagent.

Abstract translation: 一种用于检测微生物存在的诊断试剂盒，其包含不溶性底物; 和固定在不溶性底物上的碳水化合物受体，能够吸附微生物的碳水化合物受体; 和用于检测与碳水化合物受体结合的微生物的存在的标记试剂和检测样品中特定微生物的存在的方法，其包括将被检样品与固定在不溶性底物上的碳水化合物受体接触; 并通过使用标记的试剂测定样品中的微生物与碳水化合物受体的结合程度。

公开(公告)号：US20110135641A1

公开(公告)日：2011-06-09

申请号：US13057447

申请日：2009-08-05

Applicant: Jeffrey S. Isenberg ,  David D. Roberts ,  Justin Maxhimer

Inventor： Jeffrey S. Isenberg ,  David D. Roberts ,  Justin Maxhimer

IPC: A61K39/395 ,  A61K31/7088 ,  A61K38/17 ,  A61K38/08 ,  A61K38/10 ,  A61P35/00 ,  A61P39/00

CPC classification number: A61K38/39 ,  A61K2039/505 ,  A61N2005/1094 ,  C07K14/70596 ,  C07K14/78 ,  C07K16/18 ,  C07K16/2803 ,  C07K2317/70 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/3233

Abstract: Described herein is the discovery that cell and tissue survival can be dramatically increased following radiation exposure through inhibition of the interaction between TSP-1 and CD47. This effect is shown using antisense molecules, peptides, and antibodies, which can now be used as radioprotectant agents. These agents find application in minimizing, reducing and/or preventing tissue damage following intentional and accidental radiation exposure, as well as increasing the therapeutic efficacy of radiation therapies by protecting non-target tissue from incidental radiation damage and by increasing tumor ablation following radiation treatment.

Abstract translation: 本文描述的是通过抑制TSP-1和CD47之间的相互作用在辐射暴露后细胞和组织存活可以显着增加的发现。 这种效应使用反义分子，肽和抗体显示，其现在可以用作放射防护剂。 这些试剂在减少，减少和/或预防有意和意外的辐射暴露后的组织损伤中的应用，以及通过保护非目标组织免受附带的辐射损伤并通过在放射治疗之后增加肿瘤消融来增加放射疗法的治疗功效。

公开(公告)号：US07745144B2

公开(公告)日：2010-06-29

申请号：US11466718

申请日：2006-08-23

Applicant: David D. Roberts ,  Henry C. Krutzsch

Inventor： David D. Roberts ,  Henry C. Krutzsch

IPC: G01N33/53 ,  A61K38/04

CPC classification number: C07K7/06 ,  A61K38/00

Abstract: The present invention relates to a peptide comprising the sequence R1—X1—X2—X3—X4—R2, wherein X1 is selected from the group consisting of N, Q, D and S; X2 is selected from the group consisting of V, I and L; X1 is selected from the group consisting of R and K; and X4 is selected from the group consisting of V, I, L and F; R1 is a hydrogen or a peptide of 1 to 6 amino acids, and acyl or an aryl group; and R2 is a peptide of 1 to 3 amino acids, a hydroxide or an amide. The invention also relates to partial or full retro-inverso peptides comprising the above sequences. The invention also relates to peptide-substrate combination comprising a substrate suitable for cell growth and the peptide of the invention, and to a vascular graft and an artificial blood vessel comprising the peptide-substrate combination. The invention also relates to a pharmaceutical composition and a peptide conjugate comprising the peptide of the invention. The invention also relates to a method of inhibiting adhesion of a cell expressing α3β1 integrin to an extracellular matrix, inhibiting α3β1-integrin-mediated cell motility, inhibiting α3β1-integrin mediated cell proliferation, promoting α3β1-integrin mediated cell proliferation and inhibiting angiogenesis utilizing the peptides of the invention.

Abstract translation: 本发明涉及包含序列R1-X1-X2-X3-X4-R2的肽，其中X1选自N，Q，D和S; X2选自V，I和L; X1选自R和K组成的组; X4选自V，I，L和F; R1是氢或1-6个氨基酸的肽，酰基或芳基; R2为1〜3个氨基酸的肽，氢氧化物或酰胺。 本发明还涉及包含上述序列的部分或全部逆转肽。 本发明还涉及包含适合于细胞生长的底物和本发明的肽的肽 - 底物组合以及包含肽 - 底物组合的血管移植物和人造血管。 本发明还涉及包含本发明的肽的药物组合物和肽缀合物。 本发明还涉及抑制α3和bgr1整联蛋白与细胞外基质粘附的方法，抑制α3和bgr1-整联蛋白介导的细胞运动，抑制α3和bgr1-整联蛋白介导的细胞增殖，促进α3和bgr1-整联蛋白 使用本发明的肽介导的细胞增殖和抑制血管发生。

公开(公告)号：US06875855B1

公开(公告)日：2005-04-05

申请号：US09258634

申请日：1999-02-26

Applicant: David D. Roberts ,  Sizhuang Steve Yan

Inventor： David D. Roberts ,  Sizhuang Steve Yan

IPC: C07K14/40 ,  C12N15/31 ,  C07H21/04

CPC classification number: C07K14/40

Abstract: Three hemoglobin-response genes in the pathogenic yeast Candida albicans are disclosed. The expression of these genes is specifically induced when the organism is exposed to hemoglobin during disseminated infections. The invention relates to the nucleic acid and amino acid sequences of these hemoglobin-response genes. The invention also relates to diagnostic methods, kits and compositions which employ the nucleic acid and amino acid sequences of the invention.

Abstract translation: 公开了致病酵母白念珠菌中的三种血红蛋白反应基因。 当生物在播散感染期间暴露于血红蛋白时，这些基因的表达被特异性诱导。 本发明涉及这些血红蛋白应答基因的核酸和氨基酸序列。 本发明还涉及采用本发明的核酸和氨基酸序列的诊断方法，试剂盒和组合物。

公开(公告)号：US06458767B1

公开(公告)日：2002-10-01

申请号：US09321932

申请日：1999-05-28

Applicant: Joanne E. Murphy-Ullrich ,  Solange M. F. Ribeiro ,  Christian Hugo ,  David D. Roberts ,  Henry C. Krutzsch

Inventor： Joanne E. Murphy-Ullrich ,  Solange M. F. Ribeiro ,  Christian Hugo ,  David D. Roberts ,  Henry C. Krutzsch

IPC: A61K3807

CPC classification number: C07K5/1019 ,  A61K38/00 ,  C07K5/1021 ,  C07K14/78 ,  C07K16/18

Abstract: The present invention provides a method of treating kidney or renal diseases/conditions in a subject by administering to the subject a pharmaceutically effective amount of a purified LAP peptide, a TSP-1 type 1 repeat peptide, or a fragment thereof to interfere with the activation process of TGF-&bgr; by thrombospondin-1 to reduce and/or prevent renal damage. The present invention further provides a method of improving renal function in a subject having impaired renal function by administering to the subject a pharmaceutically effective amount of a purified LAP peptide, a TSP-1 type 1 repeat peptide, or a fragment thereof.

Abstract translation: 本发明提供了通过向受试者施用药学有效量的纯化的LAP肽，TSP-1型1重复肽或其片段来干扰激活的方法来治疗受试者的肾或肾脏疾病/病症 通过血小板反应蛋白-1来减少和/或预防肾损伤的TGF-β的过程。 本发明还提供了通过向受试者施用药学有效量的纯化的LAP肽，TSP-1型1重复肽或其片段，改善患有肾功能受损的受试者的肾功能的方法。