公开(公告)号：US6010889A

公开(公告)日：2000-01-04

申请号：US817290

申请日：1997-06-23

申请人： David Gearey ,  David Edward Woolley ,  Robert David Eden

发明人： David Gearey ,  David Edward Woolley ,  Robert David Eden

IPC分类号： C12M1/34 ,  C12M3/04 ,  G01N33/487 ,  C12N13/00 ,  C12Q1/02 ,  G01N27/26

CPC分类号： G01N33/4833

摘要： A method and apparatus is presented for analyzing directly obtained electrochemical noise measurements at cellular surfaces to assess cellular behavior and metabolic activity. The apparatus comprises a container (1) into which culture medium containing the cells to be assessed (2) has been introduced. Located within the container and partially submerged by the culture medium is a main electrode (3) which is a chemically inert electrically conductive surface such as gold film. This electrically conductive surface supports the adherent, viable cells to be assessed, and which may have been previously grown on the electrode to near confluence. The container is closed by a lid (4) which is penetrated by a tube (5) filled with an electrochemically conducting medium that is in electrochemical contact with a reference electrode. The end of the tube (5) is immersed in the culture medium. A further tube is provided through which test factors such as stimulants or suppressants can be injected into the container as indicated by the arrow. The directly measured electrochemical noise reflects metabolic activity of the cell at the cell surface. Fluctuations in the electrochemical noise are analyzed in the conventional manner and correlated with cellular activity.

摘要翻译： PCT No.PCT / GB95 / 02297第 371日期：1997年6月23日 102（e）日期1997年6月23日PCT提交1995年9月29日PCT公布。 公开号WO96 / 10742 日期1996年4月11日提出了一种用于分析直接获得的细胞表面的电化学噪声测量以评估细胞行为和代谢活性的方法和装置。 该装置包括容器（1），其中已经引入含有待评估细胞（2）的培养基。 位于容器内并被培养基部分浸没的是主要电极（3），其是诸如金膜的化学惰性导电表面。 该导电表面支持待评估的粘附的活细胞，并且其可以先前在电极上生长至接近汇合。 容器被盖（4）封闭，盖子（4）被填充有与参比电极电化学接触的电化学传导介质的管（5）穿透。 将管（5）的末端浸入培养基中。 提供另一个管，如箭头所示，可以将诸如兴奋剂或抑制剂的测试因子注入容器中。 直接测量的电化学噪声反映了细胞表面细胞的代谢活性。 电化学噪声的波动以常规方式分析，并与细胞活性相关。