公开(公告)号：US20050266435A1

公开(公告)日：2005-12-01

申请号：US11076431

申请日：2005-03-09

申请人： David Hackos ,  Tito Serafini ,  Nina Orike

发明人： David Hackos ,  Tito Serafini ,  Nina Orike

IPC分类号： C07K14/705 ,  C07K16/28 ,  C12N15/12 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/567 ,  G01N33/68 ,  G01N33/94

CPC分类号： G01N33/6896 ,  C07K14/705 ,  G01N33/94 ,  G01N33/9486 ,  G01N2500/00 ,  G01N2800/2842

摘要： The present invention relates to nucleic and amino acid sequences encoding vanilloid receptors which are vanilloid-insensitive. The present invention provides mutant vanilloid receptors which are insensitive to vanilloid, but which are capable of responding to low pH, heat and other receptor modulators. The invention particularly provides mutant vanilloid insensitive human VR1 receptors. The invention also relates to methods and assays for screening for vanilloid receptor modulators that act independent of the vanilloid binding site and modulate receptor signals independent of a functional vanilloid, or capsaicin, response. The invention further provides methods of modulating the vanilloid receptor, independent of vanilloid response.

摘要翻译： 本发明涉及编码香草素不敏感的香草素受体的核酸和氨基酸序列。 本发明提供对香草素不敏感但能够对低pH，热等受体调节剂作出反应的突变型香草素受体。 本发明特别提供突变型香草素不敏感人VR1受体。 本发明还涉及用于筛选不依赖于香草素结合位点的香草素受体调节剂的方法和测定，并且调节与功能性香草素或辣椒素反应无关的受体信号。 本发明进一步提供调节香草素受体的方法，与香草素反应无关。